Molecular cloning, nucleotide sequence, and expression of shl, a new gene in the 2-minute region of the genetic map of Escherichia coli

G. Leclerc, G. Noel, G. R. Drapeau

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Cells of Escherichia coli that harbor supH (an allele of the wild-type gene serU) are sensitive to UV irradiation and temperature and appera to have an impaired cell division control mechanism. We found that a gene located at the 2-min region, designated shl, inhibited the growth of supH-harboring cells when carried by a high-copy-number plasmid, whereas the same plasmid had no visible effect when present in parental cells. The amino acid sequence predicted from the nucleotide sequence of the shl gene indicated a similarity to the GalR and LacI repressor proteins, suggesting it is a transcription regulator. The sequence between the promoter and the structural genes revealed the presence of a short open reading frame of 28 amino acid residues followed by a segment of 81 base pairs. These structural features suggest that a transcription antitermination mechanism may be involved in the regulation of expression of the shl gene. The possibility that shl is a regulator of serU is discussed.

Original languageEnglish
Pages (from-to)4696-4700
Number of pages5
JournalJournal of Bacteriology
Volume172
Issue number8
StatePublished - Aug 18 1990
Externally publishedYes

Fingerprint

Molecular Cloning
Escherichia coli
Genes
Plasmids
Lac Repressors
Repressor Proteins
Gene Expression Regulation
Base Pairing
Cell Division
Open Reading Frames
Amino Acid Sequence
Alleles
Amino Acids
Temperature
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Molecular cloning, nucleotide sequence, and expression of shl, a new gene in the 2-minute region of the genetic map of Escherichia coli. / Leclerc, G.; Noel, G.; Drapeau, G. R.

In: Journal of Bacteriology, Vol. 172, No. 8, 18.08.1990, p. 4696-4700.

Research output: Contribution to journalArticle

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