Molecular cloning and expression of cDNA for a carcinoembryonic antigen-related fetal liver glycoprotein

Wasif Khan, A. Osterman, S. Hammarstrom

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Carcinoembryonic antigen (CEA) is considered to be an embryonic antigen that is reexpressed in carcinomas. However, at the molecular level little is known about fetal forms of CEA. We have studied fetal liver, which was originally considered to contain CEA. A first-trimester cDNA library from fetal liver was screened with CEA-specific probes, and a dominant cDNA clone was identified and sequenced. This 1.7-kilobase cDNA codes for a complete protein of 426 amino acids, of which 34 constitute a leader peptide. Structurally, it can be divided into four immunoglobulin-like domains homologous to CEA (N-A1-A2-B2) and a hydrophobic tail (12 residues). The A and B domains each contain two cysteines; the N domain has none. The protein has seven potential sites for asparagine-linked glycosylation. It is a form of pregnancy-specific β1-glycoprotein (PSβG) but differs from other PSβG species at the C terminus. The N and A1 domains show 45% and 51% amino acid sequence identity with the corresponding domains of the three CEA family members whose sequences have been determined. Expression studies showed that the cDNA codes for a 72-kDa glycoprotein that reacts immunologically with antisera to CEA, biliary glycoprotein I, and PSβG. The 72-kDa glycoprotein was released from the transfected cells. At least six mRNA species were identified in human tissues by using this cDNA as a probe. Genomic DNA analysis with an N-domain-specific probe indicated that the number of genes is relatively small.

Original languageEnglish
Pages (from-to)3332-3336
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number9
StatePublished - Jan 1 1989
Externally publishedYes

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Carcinoembryonic Antigen
Molecular Cloning
Glycoproteins
Complementary DNA
Liver
Pregnancy
Asparagine
First Pregnancy Trimester
Protein Sorting Signals
Gene Library
Glycosylation
varespladib methyl
Cysteine
Tail
Immune Sera
Amino Acid Sequence
Proteins
Clone Cells
Carcinoma
Antigens

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Molecular cloning and expression of cDNA for a carcinoembryonic antigen-related fetal liver glycoprotein. / Khan, Wasif; Osterman, A.; Hammarstrom, S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 9, 01.01.1989, p. 3332-3336.

Research output: Contribution to journalArticle

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