Molecular cloning and characterization of DNA sequences encoding rat and human atrial natriuretic factors

R. A. Zivin, J. H. Condra, R. A.F. Dixon

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A cDNA copy of the message encoding rat atrial natriuretic factor (ANF) has been cloned in Escherichia coli, and its nucleotide sequence was determined. ANF appears to be synthesized as a larger precursor, atrial pronatriodilatin. The cDNA has an open reading frame potentially encoding a protein of 152 amino acids, of which the first 24 amino acids strongly resemble a signal sequence. This is followed by a sequence with 80% homology to a second vasoactive protein, porcine cardiodilatin. The ANF peptide is contained in the COOH-terminal portion of the protein. The DNA sequence corresponding to human ANF is also presented and displays a high degree of homology to its rat counterpart. These data provide further evidence for the expression in cardiac atria of a multifactor system that may contribute to the regulation of blood pressure and extracellular fluid volume.

Original languageEnglish (US)
Pages (from-to)6325-6329
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number20 I
StatePublished - Jan 1 1984


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