Molecular characterization of a multipromoter gene encoding a chicken filamin protein

Catherine P. Barry, Jinling Xie, Vance Lemmon, Anthony P. Young

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We report the cloning and sequencing of cDNA encoding a chicken filamin protein. The 2,567 amino acid protein contains an NH2-terminal 267 amino acid actin-binding domain followed by a series of 24 repeating units that are each approximately 95 amino acids in length. The overall primary structure of filamin closely resembles that of human actin-binding protein (ABP). However, filamin lacks a 24-amino-acid insertion prior to repeat 16 that is contained within ABP. This region of human ABP is a site of calpain cleavage and is thought to confer flexibility on the molecule. Hence, it is possible that the properties of actin gels formed with either human ABP or filamin reflect the presence or absence of this insertion. Filamin is encoded within a multi-promoter transcription unit. A downstream filamin promoter (Fill) resembles those of certain housekeeping genes and has a putative binding site for the transcription factor E2F. Thus, transcription from this promoter may be linked to the cell cycle. A second filamin promoter (Fi12) is located at least 8 kilobases upstream from the Fill promoter. This structural arrangement suggests that regulation of filamin gene expression is likely to be complex.

Original languageEnglish
Pages (from-to)25577-25586
Number of pages10
JournalJournal of Biological Chemistry
Volume268
Issue number34
StatePublished - Dec 5 1993
Externally publishedYes

Fingerprint

Filamins
Gene encoding
Microfilament Proteins
Genes
Amino Acids
Transcription
Actins
E2F Transcription Factors
Calpain
Cloning
Essential Genes
Gene Expression Regulation
chicken filamin protein
Gene expression
Organism Cloning
Cell Cycle
Complementary DNA
Gels
Binding Sites
Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular characterization of a multipromoter gene encoding a chicken filamin protein. / Barry, Catherine P.; Xie, Jinling; Lemmon, Vance; Young, Anthony P.

In: Journal of Biological Chemistry, Vol. 268, No. 34, 05.12.1993, p. 25577-25586.

Research output: Contribution to journalArticle

Barry, Catherine P. ; Xie, Jinling ; Lemmon, Vance ; Young, Anthony P. / Molecular characterization of a multipromoter gene encoding a chicken filamin protein. In: Journal of Biological Chemistry. 1993 ; Vol. 268, No. 34. pp. 25577-25586.
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