Molecular biology

Antisense transcription in the mammalian transcriptome

S. Katayama, Y. Tomaru, T. Kasukawa, K. Waki, M. Nakanishi, M. Nakamura, H. Nishida, C. C. Yap, M. Suzuki, J. Kawai, H. Suzuki, P. Carninci, Y. Hayashizaki, C. Wells, M. Frith, T. Ravasi, K. C. Pang, J. Hallinan, J. Mattick, D. A. Hume & 11 others L. Lipovich, S. Batalov, P. G. Engström, Y. Mizuno, Mohammad A Faghihi, A. Sandelin, A. M. Chalk, S. Mottagui-Tabar, Z. Liang, B. Lenhard, Claes R Wahlestedt

Research output: Contribution to journalArticle

1149 Citations (Scopus)

Abstract

Antisense transcription (transcription from the opposite strand to a protein-coding or sense strand) has been ascribed roles in gene regulation involving degradation of the corresponding sense transcripts (RNA interference), as well as gene silencing at the chromatin level. Global transcriptome analysis provides evidence that a large proportion of the genome can produce transcripts from both strands, and that antisense transcripts commonly link neighboring "genes" in complex loci into chains of linked transcriptional units. Expression profiling reveals frequent concordant regulation of sense/antisense pairs. We present experimental evidence that perturbation of an antisense RNA can alter the expression of sense messenger RNAs, suggesting that antisense transcription contributes to control of transcriptional outputs in mammals.

Original languageEnglish
Pages (from-to)1564-1566
Number of pages3
JournalScience
Volume309
Issue number5740
DOIs
StatePublished - Sep 2 2005
Externally publishedYes

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RNA Interference
Transcriptome
Molecular Biology
Antisense RNA
Gene Silencing
Gene Expression Profiling
Genes
Chromatin
Mammals
Genome
Messenger RNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Katayama, S., Tomaru, Y., Kasukawa, T., Waki, K., Nakanishi, M., Nakamura, M., ... Wahlestedt, C. R. (2005). Molecular biology: Antisense transcription in the mammalian transcriptome. Science, 309(5740), 1564-1566. https://doi.org/10.1126/science.1112009

Molecular biology : Antisense transcription in the mammalian transcriptome. / Katayama, S.; Tomaru, Y.; Kasukawa, T.; Waki, K.; Nakanishi, M.; Nakamura, M.; Nishida, H.; Yap, C. C.; Suzuki, M.; Kawai, J.; Suzuki, H.; Carninci, P.; Hayashizaki, Y.; Wells, C.; Frith, M.; Ravasi, T.; Pang, K. C.; Hallinan, J.; Mattick, J.; Hume, D. A.; Lipovich, L.; Batalov, S.; Engström, P. G.; Mizuno, Y.; Faghihi, Mohammad A; Sandelin, A.; Chalk, A. M.; Mottagui-Tabar, S.; Liang, Z.; Lenhard, B.; Wahlestedt, Claes R.

In: Science, Vol. 309, No. 5740, 02.09.2005, p. 1564-1566.

Research output: Contribution to journalArticle

Katayama, S, Tomaru, Y, Kasukawa, T, Waki, K, Nakanishi, M, Nakamura, M, Nishida, H, Yap, CC, Suzuki, M, Kawai, J, Suzuki, H, Carninci, P, Hayashizaki, Y, Wells, C, Frith, M, Ravasi, T, Pang, KC, Hallinan, J, Mattick, J, Hume, DA, Lipovich, L, Batalov, S, Engström, PG, Mizuno, Y, Faghihi, MA, Sandelin, A, Chalk, AM, Mottagui-Tabar, S, Liang, Z, Lenhard, B & Wahlestedt, CR 2005, 'Molecular biology: Antisense transcription in the mammalian transcriptome', Science, vol. 309, no. 5740, pp. 1564-1566. https://doi.org/10.1126/science.1112009
Katayama S, Tomaru Y, Kasukawa T, Waki K, Nakanishi M, Nakamura M et al. Molecular biology: Antisense transcription in the mammalian transcriptome. Science. 2005 Sep 2;309(5740):1564-1566. https://doi.org/10.1126/science.1112009
Katayama, S. ; Tomaru, Y. ; Kasukawa, T. ; Waki, K. ; Nakanishi, M. ; Nakamura, M. ; Nishida, H. ; Yap, C. C. ; Suzuki, M. ; Kawai, J. ; Suzuki, H. ; Carninci, P. ; Hayashizaki, Y. ; Wells, C. ; Frith, M. ; Ravasi, T. ; Pang, K. C. ; Hallinan, J. ; Mattick, J. ; Hume, D. A. ; Lipovich, L. ; Batalov, S. ; Engström, P. G. ; Mizuno, Y. ; Faghihi, Mohammad A ; Sandelin, A. ; Chalk, A. M. ; Mottagui-Tabar, S. ; Liang, Z. ; Lenhard, B. ; Wahlestedt, Claes R. / Molecular biology : Antisense transcription in the mammalian transcriptome. In: Science. 2005 ; Vol. 309, No. 5740. pp. 1564-1566.
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AU - Nakamura, M.

AU - Nishida, H.

AU - Yap, C. C.

AU - Suzuki, M.

AU - Kawai, J.

AU - Suzuki, H.

AU - Carninci, P.

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AU - Wells, C.

AU - Frith, M.

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AU - Pang, K. C.

AU - Hallinan, J.

AU - Mattick, J.

AU - Hume, D. A.

AU - Lipovich, L.

AU - Batalov, S.

AU - Engström, P. G.

AU - Mizuno, Y.

AU - Faghihi, Mohammad A

AU - Sandelin, A.

AU - Chalk, A. M.

AU - Mottagui-Tabar, S.

AU - Liang, Z.

AU - Lenhard, B.

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