Molecular and cellular requirements for enhanced antigen cross-presentation to CD8 cytotoxic T lymphocytes

Satoshi Oizumi, Natasa Strbo, Savita G Pahwa, Vadim Deyev, Eckhard R. Podack

Research output: Contribution to journalArticle

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Abstract

MHC class I-mediated cross-priming of CD8 T cells by APCs is critical for CTL-based immunity to viral infections and tumors. We have shown previously that tumor-secreted heat shock protein gp96-chaperoned peptides cross prime CD8 CTL that are specific for genuine tumor Ags and for the surrogate Ag OVA. We now show that tumor-secreted heat shock protein gp96-chaperoned peptides enhance the efficiency of Ag cross-priming of CD8 CTL by several million-fold over the cross-priming activity of unchaperoned protein alone. Gp96 also acts as adjuvant for cross-priming by unchaperoned proteins, but in this capacity gp96 is 1000-fold less active than as a peptide chaperone. Mechanistically, the in situ secretion of gp96-Ig by transfected tumor cells recruits and activates dendritic cells and NK cells to the site of gp96 release and promotes CD8 CTL expansion locally. Gp96-mediated cross-priming of CD8 T cells requires B7.1/2 costimulation but proceeds unimpeded in lymph node-deficient mice, in the absence of NKT and CD4 cells and without CD40L. Gp96-driven MHC I cross-priming of CD8 CTL in the absence of lymph nodes provides a novel mechanism for local, tissue-based CTL generation at the site of gp96 release. This pathway may constitute a critically important, early detection, and rapid response mechanism that is operative in parenchymal tissues for effective defense against tissue damaging antigenic agents.

Original languageEnglish
Pages (from-to)2310-2317
Number of pages8
JournalJournal of Immunology
Volume179
Issue number4
StatePublished - Aug 15 2007

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Cross-Priming
Antigen Presentation
Cytotoxic T-Lymphocytes
Neoplasms
Heat-Shock Proteins
Peptides
Lymph Nodes
T-Lymphocytes
CD40 Ligand
Natural Killer T-Cells
Virus Diseases
Natural Killer Cells
Dendritic Cells
Immunity
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular and cellular requirements for enhanced antigen cross-presentation to CD8 cytotoxic T lymphocytes. / Oizumi, Satoshi; Strbo, Natasa; Pahwa, Savita G; Deyev, Vadim; Podack, Eckhard R.

In: Journal of Immunology, Vol. 179, No. 4, 15.08.2007, p. 2310-2317.

Research output: Contribution to journalArticle

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