Abstract
The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 409-417 |
| Number of pages | 9 |
| Journal | Analytical Biochemistry |
| Volume | 151 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1985 |
| Externally published | Yes |
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Keywords
- bovine phospholipids
- phosphatidylcholine
- phosphatidylethanolamine
- phosphatidylserine
- ROS
- stray light
- uv absorption
- uv spectrum
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane. / N'Soukpoé-Kossi, C. N.; Salesse, C.; Leblanc, Roger; Boucher, F.
In: Analytical Biochemistry, Vol. 151, No. 2, 01.01.1985, p. 409-417.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane
AU - N'Soukpoé-Kossi, C. N.
AU - Salesse, C.
AU - Leblanc, Roger
AU - Boucher, F.
PY - 1985/1/1
Y1 - 1985/1/1
N2 - The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.
AB - The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.
KW - bovine phospholipids
KW - phosphatidylcholine
KW - phosphatidylethanolamine
KW - phosphatidylserine
KW - ROS
KW - stray light
KW - uv absorption
KW - uv spectrum
UR - http://www.scopus.com/inward/record.url?scp=0022296455&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022296455&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(85)90196-4
DO - 10.1016/0003-2697(85)90196-4
M3 - Article
C2 - 4096379
AN - SCOPUS:0022296455
VL - 151
SP - 409
EP - 417
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -