Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane

C. N. N'Soukpoé-Kossi; C. Salesse; R. M. Leblanc; F. Boucher

doi:10.1016/0003-2697(85)90196-4

Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane

C. N. N'Soukpoé-Kossi, C. Salesse, R. M. Leblanc, F. Boucher

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.

Original language	English (US)
Pages (from-to)	409-417
Number of pages	9
Journal	Analytical Biochemistry
Volume	151
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(85)90196-4
State	Published - Dec 1985
Externally published	Yes

Keywords

bovine phospholipids
phosphatidylcholine
phosphatidylethanolamine
phosphatidylserine
ROS
stray light
uv absorption
uv spectrum

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/0003-2697(85)90196-4

Cite this

@article{e48b64a760fd444cad947e866b2b50e0,

title = "Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane",

abstract = "The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.",

keywords = "bovine phospholipids, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, ROS, stray light, uv absorption, uv spectrum",

author = "N'Soukpo{\'e}-Kossi, {C. N.} and C. Salesse and Leblanc, {R. M.} and F. Boucher",

note = "Funding Information: The authors thank Prof. Louise Martin for her help in computing the results. This work was supported by the Natural Sciences and Engineering Research Council of Canada and the Minis&e de 1{\textquoteright}Education du Quebec.",

year = "1985",

month = dec,

doi = "10.1016/0003-2697(85)90196-4",

language = "English (US)",

volume = "151",

pages = "409--417",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane

AU - N'Soukpoé-Kossi, C. N.

AU - Salesse, C.

AU - Leblanc, R. M.

AU - Boucher, F.

N1 - Funding Information: The authors thank Prof. Louise Martin for her help in computing the results. This work was supported by the Natural Sciences and Engineering Research Council of Canada and the Minis&e de 1’Education du Quebec.

PY - 1985/12

Y1 - 1985/12

N2 - The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.

AB - The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.

KW - bovine phospholipids

KW - phosphatidylcholine

KW - phosphatidylethanolamine

KW - phosphatidylserine

KW - ROS

KW - stray light

KW - uv absorption

KW - uv spectrum

UR - http://www.scopus.com/inward/record.url?scp=0022296455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022296455&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(85)90196-4

DO - 10.1016/0003-2697(85)90196-4

M3 - Article

C2 - 4096379

AN - SCOPUS:0022296455

VL - 151

SP - 409

EP - 417

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -

Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this