Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane

C. N. N'Soukpoé-Kossi, C. Salesse, Roger Leblanc, F. Boucher

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.

Original languageEnglish
Pages (from-to)409-417
Number of pages9
JournalAnalytical Biochemistry
Volume151
Issue number2
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Rod Cell Outer Segment
Retina
Phospholipids
Stray light
Lipids
Light
Wavelength
Chemical analysis
n-hexane

Keywords

  • bovine phospholipids
  • phosphatidylcholine
  • phosphatidylethanolamine
  • phosphatidylserine
  • ROS
  • stray light
  • uv absorption
  • uv spectrum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane. / N'Soukpoé-Kossi, C. N.; Salesse, C.; Leblanc, Roger; Boucher, F.

In: Analytical Biochemistry, Vol. 151, No. 2, 01.01.1985, p. 409-417.

Research output: Contribution to journalArticle

N'Soukpoé-Kossi, C. N. ; Salesse, C. ; Leblanc, Roger ; Boucher, F. / Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane. In: Analytical Biochemistry. 1985 ; Vol. 151, No. 2. pp. 409-417.
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