Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane

C. N. N'Soukpoé-Kossi, C. Salesse, R. M. Leblanc, F. Boucher

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.

Original languageEnglish (US)
Pages (from-to)409-417
Number of pages9
JournalAnalytical Biochemistry
Volume151
Issue number2
DOIs
StatePublished - Dec 1985
Externally publishedYes

Keywords

  • bovine phospholipids
  • phosphatidylcholine
  • phosphatidylethanolamine
  • phosphatidylserine
  • ROS
  • stray light
  • uv absorption
  • uv spectrum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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