Abstract
The molar absorptivities of the major bovine rod outer segment (ROS) phospholipids and phophatidylcholine 18:1 have been determined at four wavelengths, i.e., 193.5, 196, 200, and 205 nm. The mean standard error is 1.7% at 95% confidence level. The results obtained can therefore be used for the quantitative analysis of bovine ROS phospholipids. Compared with the existing methods, the spectrophotometric determination of these lipids presents the advantages of being rapid, direct, and very sensitive. The importance of stray light in this type of measurement is also discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 409-417 |
| Number of pages | 9 |
| Journal | Analytical Biochemistry |
| Volume | 151 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 1985 |
| Externally published | Yes |
Keywords
- bovine phospholipids
- phosphatidylcholine
- phosphatidylethanolamine
- phosphatidylserine
- ROS
- stray light
- uv absorption
- uv spectrum
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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N'Soukpoé-Kossi, C. N., Salesse, C., Leblanc, R. M., & Boucher, F. (1985). Molar absorptivities of bovine retina rod outer segment phospholipids in n-hexane. Analytical Biochemistry, 151(2), 409-417. https://doi.org/10.1016/0003-2697(85)90196-4