Modulation of Wnt signaling is essential for the differentiation of ciliated epithelial cells in human airways

Andreas Schmid, Juliette Sailland, Lisa Novak, Nathalie Baumlin, Nevis L. Fregien, Matthias A Salathe

Research output: Contribution to journalLetter

8 Scopus citations

Abstract

Wnt signaling is essential for the differentiation of airway epithelial cells during development. Here, we examined the role of Wnt signaling during redifferentiation of ciliated airway epithelial cells in vitro at the air liquid interface as a model of airway epithelial repair. Phases of proliferation and differentiation were defined. Markers of squamous metaplasia and epithelial ciliation were followed while enhancing β-catenin signaling by blocking glycogen synthase kinase 3β with SB216763 and shRNA as well as inhibiting canonical WNT signaling with apical application of Dickkopf 1 (Dkk1). Our findings indicate that enhanced β-catenin signaling decreases the number of ciliated cells and causes squamous changes in the epithelium, whereas treatment with DDk1 leads to an increased number of ciliated cells.

Original languageEnglish (US)
Pages (from-to)3493-3506
Number of pages14
JournalFEBS Letters
Volume591
Issue number21
DOIs
StatePublished - Nov 1 2017

Keywords

  • airway epithelium
  • ciliated cells
  • Dickkopf
  • differentiation
  • Wnt signaling

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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