Modulation of the expression of an apical plasma membrane protein of Madin-Darby canine kidney epithelial cells: Cell-cell interactions control the appearance of a novel intracellular storage compartment

D. E. Vega-Salas, Pedro J Salas, E. Rodriguez-Boulan

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Experimental conditions that abolish or reduce to a minimum intercellular contacts between Madin-Darby canine kidney epithelial cells result in the appearance of an intracellular storage compartment for apical membrane proteins. Subconfluent culture, incubation in 1-5 μM Ca++, or inclusion of dissociated cells within agarose or collagen gels all caused the intracellular accumulation of a 184-kD apical membrane protein within large (0.5-5 μm) vacuoles, rich in microvilli. Influenza virus hemagglutinin, an apically targeted viral glycoprotein, is concentrated within these structures but the basolateral glycoprotein G of vesicular stomatitis virus and a cellular basolateral 63-kD membrane protein of Madin-Darby canine kidney cells were excluded. This novel epithelial organelle (VAC), which we designate the vacuolar apical compartment, may play an as yet unrecognized role in the biogenesis of the apical plasma membrane during the differentiation of normal epithelia.

Original languageEnglish
Pages (from-to)1249-1259
Number of pages11
JournalJournal of Cell Biology
Volume104
Issue number5
StatePublished - Jan 1 1987
Externally publishedYes

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Madin Darby Canine Kidney Cells
Cell Communication
Blood Proteins
Membrane Proteins
Epithelial Cells
Cell Membrane
Glycoproteins
Vesicular Stomatitis
Hemagglutinins
Microvilli
Vacuoles
Orthomyxoviridae
Organelles
Sepharose
Collagen
Epithelium
Gels
Viruses

ASJC Scopus subject areas

  • Cell Biology

Cite this

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