Modulation of rat lung Na+,K+-ATPase gene expression by hyperoxia

C. R. Johnson, Y. Guo, E. S. Helton, S. Matalon, Robert Jackson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rats exposed to 85% O2 for 5-7 days develop tolerance to otherwise lethal hyperoxia (100% O2). The rate of alveolar fluid clearance increases during adaptation to hyperoxia, due in part to increased alveolar epithelial sodium channel activity. In these studies, we have investigated molecular mechanisms leading to increased lung Na+,K+-ATPase activity in hyperoxia. We exposed adult rats to 85% O2 (sublethal hyperoxia) for 7 days, followed by 2, 3, or 4 days in 100% O2. Steady-state levels of the Na+,K+-ATPase α 1 and β1 subunit mRNAs increased in whole lung tissue during hyperoxia exposures. Stability of the Na+,K+-ATPase α1 and β1 subunit mRNA messages in whole lung RNA did not change significantly. Thus, lung Na+,K+-ATPase gene expression in sublethal hyperoxia appears to be regulated in part at the transcriptional level. Alveolar epithelial type II (ATII) cell Na+,K+- ATPase α1 and β1 subunit proteins, measured by quantitative immunofluorescence, increased significantly after sublethal hyperoxia and 100% O2 exposures. Increases in lung fluid clearance after sublethal hyperoxia are associated with increased ATII cell Na+,K+-ATPase protein and whole lung Na+,K+-ATPase mRNA expression, which correspond to previously described increases in epithelial sodium channel expression under these conditions.

Original languageEnglish
Pages (from-to)173-188
Number of pages16
JournalExperimental Lung Research
Volume24
Issue number2
StatePublished - Apr 20 1998
Externally publishedYes

Fingerprint

Hyperoxia
Gene expression
Adenosine Triphosphatases
Rats
Modulation
Gene Expression
Lung
Epithelial Sodium Channels
Alveolar Epithelial Cells
Messenger RNA
Fluids
Protein Subunits
sodium-translocating ATPase
Fluorescent Antibody Technique
RNA
Tissue

Keywords

  • Alveolar epithelium
  • Na,K-ATPase
  • Oxygen toxicity
  • Pulmonary edema
  • Sodium transport

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Johnson, C. R., Guo, Y., Helton, E. S., Matalon, S., & Jackson, R. (1998). Modulation of rat lung Na+,K+-ATPase gene expression by hyperoxia. Experimental Lung Research, 24(2), 173-188.

Modulation of rat lung Na+,K+-ATPase gene expression by hyperoxia. / Johnson, C. R.; Guo, Y.; Helton, E. S.; Matalon, S.; Jackson, Robert.

In: Experimental Lung Research, Vol. 24, No. 2, 20.04.1998, p. 173-188.

Research output: Contribution to journalArticle

Johnson, CR, Guo, Y, Helton, ES, Matalon, S & Jackson, R 1998, 'Modulation of rat lung Na+,K+-ATPase gene expression by hyperoxia', Experimental Lung Research, vol. 24, no. 2, pp. 173-188.
Johnson CR, Guo Y, Helton ES, Matalon S, Jackson R. Modulation of rat lung Na+,K+-ATPase gene expression by hyperoxia. Experimental Lung Research. 1998 Apr 20;24(2):173-188.
Johnson, C. R. ; Guo, Y. ; Helton, E. S. ; Matalon, S. ; Jackson, Robert. / Modulation of rat lung Na+,K+-ATPase gene expression by hyperoxia. In: Experimental Lung Research. 1998 ; Vol. 24, No. 2. pp. 173-188.
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