Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture

N. Štrbo, G. Laškarin, V. Sotošek, L. J. Randić, E. R. Podack, D. Rukavina

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.

Original languageEnglish (US)
Pages (from-to)14-21
Number of pages8
JournalAmerican Journal of Reproductive Immunology
Volume42
Issue number1
DOIs
StatePublished - Jul 1999

Keywords

  • Decidual lymphocytes
  • Decidual stromal cells
  • First trimester pregnancy
  • Perforin
  • Peripheral blood lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

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