Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture

Natasa Strbo, G. Laškarin, V. Sotošek, L. J. Randić, E. R. Podack, D. Rukavina

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.

Original languageEnglish
Pages (from-to)14-21
Number of pages8
JournalAmerican Journal of Reproductive Immunology
Volume42
Issue number1
StatePublished - Jul 1 1999
Externally publishedYes

Fingerprint

Perforin
Lymphocytes
Decidua
Pregnancy
First Pregnancy Trimester
Mitogens
Suspensions
Flow Cytometry
Up-Regulation
Down-Regulation
Antigens

Keywords

  • Decidual lymphocytes
  • Decidual stromal cells
  • First trimester pregnancy
  • Perforin
  • Peripheral blood lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Obstetrics and Gynecology

Cite this

Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture. / Strbo, Natasa; Laškarin, G.; Sotošek, V.; Randić, L. J.; Podack, E. R.; Rukavina, D.

In: American Journal of Reproductive Immunology, Vol. 42, No. 1, 01.07.1999, p. 14-21.

Research output: Contribution to journalArticle

Strbo, N, Laškarin, G, Sotošek, V, Randić, LJ, Podack, ER & Rukavina, D 1999, 'Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture', American Journal of Reproductive Immunology, vol. 42, no. 1, pp. 14-21.
Strbo, Natasa ; Laškarin, G. ; Sotošek, V. ; Randić, L. J. ; Podack, E. R. ; Rukavina, D. / Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture. In: American Journal of Reproductive Immunology. 1999 ; Vol. 42, No. 1. pp. 14-21.
@article{e81fb2c32195405a99ab0b513feeb2cd,
title = "Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture",
abstract = "PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30{\%} of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.",
keywords = "Decidual lymphocytes, Decidual stromal cells, First trimester pregnancy, Perforin, Peripheral blood lymphocytes",
author = "Natasa Strbo and G. Laškarin and V. Sotošek and Randić, {L. J.} and Podack, {E. R.} and D. Rukavina",
year = "1999",
month = "7",
day = "1",
language = "English",
volume = "42",
pages = "14--21",
journal = "American Journal of Reproductive Immunology and Microbiology",
issn = "1046-7408",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture

AU - Strbo, Natasa

AU - Laškarin, G.

AU - Sotošek, V.

AU - Randić, L. J.

AU - Podack, E. R.

AU - Rukavina, D.

PY - 1999/7/1

Y1 - 1999/7/1

N2 - PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.

AB - PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.

KW - Decidual lymphocytes

KW - Decidual stromal cells

KW - First trimester pregnancy

KW - Perforin

KW - Peripheral blood lymphocytes

UR - http://www.scopus.com/inward/record.url?scp=0344052675&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344052675&partnerID=8YFLogxK

M3 - Article

C2 - 10429762

AN - SCOPUS:0344052675

VL - 42

SP - 14

EP - 21

JO - American Journal of Reproductive Immunology and Microbiology

JF - American Journal of Reproductive Immunology and Microbiology

SN - 1046-7408

IS - 1

ER -