Abstract
PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.
| Original language | English |
|---|---|
| Pages (from-to) | 14-21 |
| Number of pages | 8 |
| Journal | American Journal of Reproductive Immunology |
| Volume | 42 |
| Issue number | 1 |
| State | Published - Jul 1 1999 |
| Externally published | Yes |
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Keywords
- Decidual lymphocytes
- Decidual stromal cells
- First trimester pregnancy
- Perforin
- Peripheral blood lymphocytes
ASJC Scopus subject areas
- Immunology
- Obstetrics and Gynecology
Cite this
Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture. / Strbo, Natasa; Laškarin, G.; Sotošek, V.; Randić, L. J.; Podack, E. R.; Rukavina, D.
In: American Journal of Reproductive Immunology, Vol. 42, No. 1, 01.07.1999, p. 14-21.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Modulation of perforin expression in the decidual and peripheral blood cytotoxic lymphocytes in culture
AU - Strbo, Natasa
AU - Laškarin, G.
AU - Sotošek, V.
AU - Randić, L. J.
AU - Podack, E. R.
AU - Rukavina, D.
PY - 1999/7/1
Y1 - 1999/7/1
N2 - PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.
AB - PROBLEM: Perforin (P) is a cytolytic molecule located in intracellular granules of cytotoxic lymphocytes both in the peripheral blood and decidua of pregnancy. The aim was to analyze the kinetics of P expression during in vitro culture and modulation of P expression by adherent cells, their supernatants and mitogen (PHA) stimulation. METHOD OF STUDY: P (intracellular antigen) was detected by flow cytometry in the suspension of first trimester pregnancy peripheral blood lymphocytes (PBL) and decidual lymphocytes (DL). RESULTS: A decrease of the percentage of P+ cells was obtained after 1 hr incubation and was prevented by addition of 30% of decidual adherent cells (DAC) or their supernatants. Upregulation of P expression was obtained when, in addition to adherent cells, DL and PBL were stimulated by PHA. DAC present in the culture in physiological concentrations prevent downregulation of P expression. CONCLUSION: DAC located in the vicinity of decidual cytotoxic lymphocytes, owing to their unique ability to produce a wide range of substances on demand, contribute to the high level of P expression in the decidua of pregnancy.
KW - Decidual lymphocytes
KW - Decidual stromal cells
KW - First trimester pregnancy
KW - Perforin
KW - Peripheral blood lymphocytes
UR - http://www.scopus.com/inward/record.url?scp=0344052675&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0344052675&partnerID=8YFLogxK
M3 - Article
C2 - 10429762
AN - SCOPUS:0344052675
VL - 42
SP - 14
EP - 21
JO - American Journal of Reproductive Immunology and Microbiology
JF - American Journal of Reproductive Immunology and Microbiology
SN - 1046-7408
IS - 1
ER -