Modulation of HIV-1 replication by RNA interference

Jean Marc Jacque, Karine Triques, Mario Stevenson

Research output: Contribution to journalArticle

738 Citations (Scopus)

Abstract

RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-nucleotide duplexes of small interfering RNA (siRNA). Here we describe inhibition of early and late steps of HIV-1 replication in human cell lines and primary lymphocytes by siRNAs targeted to various regions of the HIV-1 genome. We demonstrate that synthetic siRNA duplexes or plasmid-derived siRNAs inhibit HIV-1 infection by specifically degrading genomic HIV-1 RNA, thereby preventing formation of viral complementary-DNA intermediates. These results demonstrate the utility of RNAi for modulating the HIV replication cycle and provide evidence that genomic HIV-1 RNA, as it exists within a nucleoprotein reverse-transcription complex, is amenable to siRNA-mediated degradation.

Original languageEnglish
Pages (from-to)435-438
Number of pages4
JournalNature
Volume418
Issue number6896
DOIs
StatePublished - Jul 25 2002
Externally publishedYes

Fingerprint

RNA Interference
HIV-1
Small Interfering RNA
RNA
Nucleoproteins
Double-Stranded RNA
Viral DNA
RNA Stability
Plant Cells
Reverse Transcription
HIV Infections
Plasmids
Nucleotides
Complementary DNA
HIV
Genome
Lymphocytes
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • General

Cite this

Modulation of HIV-1 replication by RNA interference. / Jacque, Jean Marc; Triques, Karine; Stevenson, Mario.

In: Nature, Vol. 418, No. 6896, 25.07.2002, p. 435-438.

Research output: Contribution to journalArticle

Jacque, Jean Marc ; Triques, Karine ; Stevenson, Mario. / Modulation of HIV-1 replication by RNA interference. In: Nature. 2002 ; Vol. 418, No. 6896. pp. 435-438.
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