Modulation of cell proliferation and gene expression by α-tocopheryl phosphates: Relevance to atherosclerosis and inflammation

Adelina Munteanu, Jean Marc Zingg, Esra Ogru, Roksan Libinaki, Robert Gianello, Simon West, Yesim Negis, Angelo Azzi

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

The effect of a mixture of α-tocopheryl phosphate and di-α-tocopheryl phosphate (TPm) was studied in vitro on two cell lines, RASMC (from rat aortic smooth muscle) and human THP-1 monocytic leukaemia cells. Inhibition of cell proliferation by TPm was shown in both lines and occurred with TPm at concentrations lower than those at which α-tocopherol was equally inhibitory. TPm led in non-stimulated THP-1 cells to inhibition of CD36 mRNA and protein expression, to inhibition of oxidized low density lipoprotein surface binding and oxLDL uptake. In non-stimulated THP-1 cells, α-tocopherol had only very weak effects on these events. Contrary to α-tocopherol, TPm was cytotoxic to THP-1 cells at high concentrations. Thus, TPm is able to inhibit the major aggravating elements involved in the progression of atherosclerosis. The higher potency of TPm may be due to a better uptake of the molecule and to its intracellular hydrolysis, providing more α-tocopherol to sensitive sites. Alternatively, a direct effect of the phosphate ester on specific cell targets may be considered.

Original languageEnglish (US)
Pages (from-to)311-316
Number of pages6
JournalBiochemical and biophysical research communications
Volume318
Issue number1
DOIs
StatePublished - May 21 2004
Externally publishedYes

Keywords

  • Apoptosis
  • CD36
  • Growth inhibition
  • OxLDL
  • Tocopherol
  • Tocopheryl phosphate

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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