Modified fibrin hydrogels stimulate angiogenesis in vivo: Potential application to increase perfusion of ischemic tissues

H. Hall, J. A. Hubbell

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The lack of angiogenesis in ischemic tissues is a major health problem and many studies aim to explore strategies to locally increase blood perfusion. Our approach is to use covalently modified fibrin-based hydrogels as a matrix that induces endothelial cell survival in vitro and angiogenesis in vivo. Fibrin hydrogels were covalently modified by L1Ig6, a specific receptor for cell survival integrin αvβ3 that is expressed on angiogenic endothelial cells. In addition, L1Ig6-modified matrices were filled with growth factors VEGF-A165 or bFGF. These hydrogels were applied on growing shell-free chicken chorioallantoic membranes (CAMs) and the developing vasculature was found to be increased by ∼50%. Moreover, the increase in αv-integrin levels in the CAMs underlying the hydrogel implants were investigated and found to be increased by ∼40% and ∼100%, respectively, after CAM stimulation with L1Ig6 alone or in combination with growth factors VEGF-A165 and bFGF. Therefore, modified fibrin hydrogels provide an interesting way to design an implant that can be introduced at the site of ischemia, and provides a scaffold and release system for growth factors that induce specific tissue responses.

Original languageEnglish (US)
Pages (from-to)768-774
Number of pages7
JournalMaterialwissenschaft und Werkstofftechnik
Volume36
Issue number12
DOIs
StatePublished - Dec 1 2005

Keywords

  • αVβ3 integrin
  • Angiogenesis
  • CAM assay
  • Endothelial cells
  • HUVECs
  • L1ig6
  • NFκB

ASJC Scopus subject areas

  • Materials Science(all)
  • Biotechnology
  • Biochemistry

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