Modification of islet of langerhans surfaces with immunoprotective poly(ethylene glycol) coatings via inferfacial photopolymerization

A. S. Sawhney, P. Pathak, J. A. Hubbell

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Poly(ethylene glycol) (PEG) has been used previously to alter immune interactions and systemic clearance of therapeutic proteins. We present herein chemical approaches for the conceptually similar treatment of therapeutic cells and tissues whereby immune and cell adhesive interactions may be reduced or interrupted, in the context of the transplantation of xenogeneic islets of Langerhans for the treatment of insulin-dependent diabetes mellitus. Visible-light-initiated interfacial photopolymerization of multifunctional PEG-based macromers was performed directly upon the surface of rat islets of Langerhans to produce conformal barrier hydrogel coatings with thickness of order 10 μm. The islets continued to be normal in ultrastructure and function as reflected by response to a glucose challenge in vitro.

Original languageEnglish
Pages (from-to)383-386
Number of pages4
JournalBiotechnology and Bioengineering
Volume44
Issue number3
DOIs
StatePublished - Jul 1 1994
Externally publishedYes

Fingerprint

Ethylene Glycol
Photopolymerization
Islets of Langerhans
Polyethylene glycols
Coatings
Hydrogel
Insulin
Medical problems
Hydrogels
Islets of Langerhans Transplantation
Glucose
Rats
Adhesives
Therapeutics
Type 1 Diabetes Mellitus
Cells
Cell Communication
Tissue
Proteins
Light

Keywords

  • Diabetes mellitus
  • Insulin
  • Islet of langerhans
  • PEG coating

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Modification of islet of langerhans surfaces with immunoprotective poly(ethylene glycol) coatings via inferfacial photopolymerization. / Sawhney, A. S.; Pathak, P.; Hubbell, J. A.

In: Biotechnology and Bioengineering, Vol. 44, No. 3, 01.07.1994, p. 383-386.

Research output: Contribution to journalArticle

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