Modern strategy of tumor suppressor gene cloning: B-cell chronic lymphocytic leukemia candidate genes search as an illustration

A. V. Baranova, D. V. Ivanov, N. V. Makeeva, E. A. Nikitin, O. Sangfeldt, T. V. Borodina, B. I. Kapanadze, T. A. Tyazhelova, M. Corcoran, L. I. Fedorova, D. V. Shagin, M. V. Nemtsova, A. B. Poltaraus, A. V. Zelenin, D. V. Zaletaev, S. A. Lukyanov, D. Grander, S. Einhorn, N. K. Yankovsky

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Genes that normally function to prevent or suppress malignancy are known as "tumor suppressor genes" or TSG. It is supposed that the chromosomal region lost in the tumor cells harbors TSG preventing cancer. The examination of this region in the normal chromosome would permit identification of the particular suppressor gene. The methods employed in such search are diverse. As a rule, success is achieved by combining cytogenetic, genetic and physical genome mapping with analysis of genomic and cDNA clones. We have constructed a cosmid contig in the 13q14.3 region which is expected to contain a putative B-cell chronic lymphocytic leukemia (BCLL) TSG. cDNA clones corresponding to new human gene Leu5 have been found to locate nearby the borders of homo-and hemizygous deletions in BCLL patients. Gene Leu5 encodes a zinc-finger protein that shares homology with some mammalian genes taking part in early embryogenesis and tumor progression. Leu5 gene could be an interesting candicate for BCLL TSG.

Original languageEnglish (US)
Pages (from-to)15-19
Number of pages5
JournalGematologiya i Transfusiologiya
Volume45
Issue number3
StatePublished - Dec 1 2000
Externally publishedYes

Keywords

  • B cell chronic lymphocytic leukemia
  • Gene mapping
  • Leu5
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Hematology

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  • Cite this

    Baranova, A. V., Ivanov, D. V., Makeeva, N. V., Nikitin, E. A., Sangfeldt, O., Borodina, T. V., Kapanadze, B. I., Tyazhelova, T. A., Corcoran, M., Fedorova, L. I., Shagin, D. V., Nemtsova, M. V., Poltaraus, A. B., Zelenin, A. V., Zaletaev, D. V., Lukyanov, S. A., Grander, D., Einhorn, S., & Yankovsky, N. K. (2000). Modern strategy of tumor suppressor gene cloning: B-cell chronic lymphocytic leukemia candidate genes search as an illustration. Gematologiya i Transfusiologiya, 45(3), 15-19.