Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute

C. L. Stabler, Christopher Fraker, E. Pedraza, I. Constantinidis, A. Sambanis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study investigated the model-based design, fabrication and in vitro and in vivo experimental characterization of a pancreatic substitute consisting of mouse insulinoma cells encapsulated in agarose in a disk-shaped construct. Two construct prototypes were examined: (i) a single disk construct comprised of agarose and βTC3 cells; and (ii) a buffered disk construct, consisting of agarose and βTC3 cells, coated with an additional layer of pure agarose. Diffusional studies of glucose and insulin were performed to characterize the transport properties of the material. Three dimensional oxygen diffusion-reaction models were used to predict the appropriate cell loadings for the two construct prototypes under varying external oxygen tensions. In vitro and in vivo experiments found the overall viable cell number for each construct prototype plateaued to the same value, regardless of the initial cell seeding number, when constructs were placed under identical environmental conditions. Furthermore, mathematical model calculations correlated well with experimental in vitro and in vivo results of cell viability, indicating oxygen tension to be the dominating factor in establishing total viable cell number in these constructs. These results indicate that modeling is useful for the development of tissue engineered constructs when permissive matrices and continuous cell lines are used. The applicability of this modeling and experimental methodology in the development of agarose-based constructs for use as a bioartificial pancreas is discussed.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages185-196
Number of pages12
Volume953
DOIs
StatePublished - Dec 1 2007
EventConference on Data Mining, Systems Analysis, and Optimization in Biomedicine, 2007 - Gainesville, FL, United States
Duration: Mar 28 2007Mar 30 2007

Other

OtherConference on Data Mining, Systems Analysis, and Optimization in Biomedicine, 2007
CountryUnited States
CityGainesville, FL
Period3/28/073/30/07

Fingerprint

substitutes
cells
oxygen tension
prototypes
pancreas
insulin
inoculation
cultured cells
viability
glucose
mice
mathematical models
transport properties
methodology
fabrication
oxygen
matrices

Keywords

  • βTC3 insulinoma cells
  • Agarose
  • Encapsulated cells
  • Pancreatic substitute
  • Tissue construct modeling

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Stabler, C. L., Fraker, C., Pedraza, E., Constantinidis, I., & Sambanis, A. (2007). Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute. In AIP Conference Proceedings (Vol. 953, pp. 185-196) https://doi.org/10.1063/1.2817341

Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute. / Stabler, C. L.; Fraker, Christopher; Pedraza, E.; Constantinidis, I.; Sambanis, A.

AIP Conference Proceedings. Vol. 953 2007. p. 185-196.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Stabler, CL, Fraker, C, Pedraza, E, Constantinidis, I & Sambanis, A 2007, Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute. in AIP Conference Proceedings. vol. 953, pp. 185-196, Conference on Data Mining, Systems Analysis, and Optimization in Biomedicine, 2007, Gainesville, FL, United States, 3/28/07. https://doi.org/10.1063/1.2817341
Stabler CL, Fraker C, Pedraza E, Constantinidis I, Sambanis A. Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute. In AIP Conference Proceedings. Vol. 953. 2007. p. 185-196 https://doi.org/10.1063/1.2817341
Stabler, C. L. ; Fraker, Christopher ; Pedraza, E. ; Constantinidis, I. ; Sambanis, A. / Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute. AIP Conference Proceedings. Vol. 953 2007. pp. 185-196
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