Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute

Cherie Stabler, C. Fraker, E. Pedraza, I. Constantinidis, A. Sambanis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This study investigated the model-based design, fabrication and in vitro and in vivo experimental characterization of a pancreatic substitute consisting of mouse insulinoma cells encapsulated in agarose in a disk-shaped construct. Two construct prototypes were examined: (i) a single disk construct comprised of agarose and βTC3 cells; and (ii) a buffered disk construct, consisting of agarose and βTC3 cells, coated with an additional layer of pure agarose. Diffusional studies of glucose and insulin were performed to characterize the transport properties of the material. Three dimensional oxygen diffusion-reaction models were used to predict the appropriate cell loadings for the two construct prototypes under varying external oxygen tensions. In vitro and in vivo experiments found the overall viable cell number for each construct prototype plateaued to the same value, regardless of the initial cell seeding number, when constructs were placed under identical environmental conditions. Furthermore, mathematical model calculations correlated well with experimental in vitro and in vivo results of cell viability, indicating oxygen tension to be the dominating factor in establishing total viable cell number in these constructs. These results indicate that modeling is useful for the development of tissue engineered constructs when permissive matrices and continuous cell lines are used. The applicability of this modeling and experimental methodology in the development of agarose-based constructs for use as a bioartificial pancreas is discussed.

Original languageEnglish (US)
Title of host publicationData Mining, Systems Analysis, and Optimization in Biomedicine
Pages185-196
Number of pages12
DOIs
StatePublished - Dec 1 2007
EventConference on Data Mining, Systems Analysis, and Optimization in Biomedicine, 2007 - Gainesville, FL, United States
Duration: Mar 28 2007Mar 30 2007

Publication series

NameAIP Conference Proceedings
Volume953
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

OtherConference on Data Mining, Systems Analysis, and Optimization in Biomedicine, 2007
CountryUnited States
CityGainesville, FL
Period3/28/073/30/07

Keywords

  • βTC3 insulinoma cells
  • Agarose
  • Encapsulated cells
  • Pancreatic substitute
  • Tissue construct modeling

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute'. Together they form a unique fingerprint.

  • Cite this

    Stabler, C., Fraker, C., Pedraza, E., Constantinidis, I., & Sambanis, A. (2007). Modeling and in vitro and in vivo characterization of a tissue engineered pancreatic substitute. In Data Mining, Systems Analysis, and Optimization in Biomedicine (pp. 185-196). (AIP Conference Proceedings; Vol. 953). https://doi.org/10.1063/1.2817341