Model systems for the study of estrogen action in tissue culture

Marc Lippman, Gail Bolan, Marie Monaco, Lawrence Pinkus, Linda Engel

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Physiologic concentrations of estrogen stimulate precursor incorporation and growth in several lines of human breast cancer cells in long term tissue culture. Antiestrogens inhibit precursor incorporation and eventually kill the cells. Estrogens reverse antiestrogen effects. Responsive cell lines contain high affinity specific cytoplasmic receptors for estrogen. When binding and stimulation are measured under similar conditions, it appears that only a small proportion of receptor sites need be occupied for maximal stimulation. Thymidine kinase specific activity is increased by estradiol and may, in part, explain enhanced thymidine incorporation. These cell lines should prove useful for the study of the mechanisms by which estrogens regulate growth in human breast cancer.

Original languageEnglish (US)
Pages (from-to)1045-1051
Number of pages7
JournalJournal of Steroid Biochemistry
Volume7
Issue number11-12
DOIs
StatePublished - Jan 1 1976

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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    Lippman, M., Bolan, G., Monaco, M., Pinkus, L., & Engel, L. (1976). Model systems for the study of estrogen action in tissue culture. Journal of Steroid Biochemistry, 7(11-12), 1045-1051. https://doi.org/10.1016/0022-4731(76)90032-7