TY - JOUR
T1 - Model systems for the study of estrogen action in tissue culture
AU - Lippman, Marc
AU - Bolan, Gail
AU - Monaco, Marie
AU - Pinkus, Lawrence
AU - Engel, Linda
PY - 1976/1/1
Y1 - 1976/1/1
N2 - Physiologic concentrations of estrogen stimulate precursor incorporation and growth in several lines of human breast cancer cells in long term tissue culture. Antiestrogens inhibit precursor incorporation and eventually kill the cells. Estrogens reverse antiestrogen effects. Responsive cell lines contain high affinity specific cytoplasmic receptors for estrogen. When binding and stimulation are measured under similar conditions, it appears that only a small proportion of receptor sites need be occupied for maximal stimulation. Thymidine kinase specific activity is increased by estradiol and may, in part, explain enhanced thymidine incorporation. These cell lines should prove useful for the study of the mechanisms by which estrogens regulate growth in human breast cancer.
AB - Physiologic concentrations of estrogen stimulate precursor incorporation and growth in several lines of human breast cancer cells in long term tissue culture. Antiestrogens inhibit precursor incorporation and eventually kill the cells. Estrogens reverse antiestrogen effects. Responsive cell lines contain high affinity specific cytoplasmic receptors for estrogen. When binding and stimulation are measured under similar conditions, it appears that only a small proportion of receptor sites need be occupied for maximal stimulation. Thymidine kinase specific activity is increased by estradiol and may, in part, explain enhanced thymidine incorporation. These cell lines should prove useful for the study of the mechanisms by which estrogens regulate growth in human breast cancer.
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U2 - 10.1016/0022-4731(76)90032-7
DO - 10.1016/0022-4731(76)90032-7
M3 - Article
C2 - 1025348
AN - SCOPUS:0017038404
VL - 7
SP - 1045
EP - 1051
JO - Journal of Steroid Biochemistry and Molecular Biology
JF - Journal of Steroid Biochemistry and Molecular Biology
SN - 0960-0760
IS - 11-12
ER -