Mobilization of peripheral blood stem cells with paclitaxel and rhG-CSF in high-risk breast cancer patients

Kenneth R. Meehan, Rebecca Slack, Edmund Gehan, Herbert B. Herscowitz, Ellen M. Areman, Mark Ebadi, Mitchell S. Cairo, Marc E. Lippman

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4 Scopus citations

Abstract

Preclinical studies have demonstrated the rapid and efficient mobilization of hematopoietic peripheral blood stem cells (PBSC) in a mouse model using the combination of paclitaxel with recombinant human granulocyte colony-stimulating factor (rhG-CSF). On the basis of these results, a clinical trial was initiated using rhG-CSF with paclitaxel for PBSC mobilization in high-risk breast cancer patients. The mobilized PBSC were evaluated for CD34+ cell number, mononuclear cell content, and clonogenic potential. One-hundred and seventeen breast cancer patients received paclitaxel (300 mg/m2) administered as a 24-h continuous intravenous infusion. Forty-eight hours after completing paclitaxel, rhG-CSF (5 μg/kg) was initiated and continued until completion of PBSC collection. Leukapheresis was initiated once the white blood cell count reached 1.0 × 109/L. Each collection was evaluated for the numbers of mononuclear cells (MNC) and CD34+ cells. Clonogenic potential was enumerated using colony-forming units-granulocyte-macrophage (CFU-GM) and burst-forming units-erythroid (BFU-E). Patients receiving paclitaxel with rhG-CSF mobilized a large number of mononuclear cells/apheresis (mean, 3.7 × 108; range, 3.3-4.1) and CD34+ cells/apheresis (mean, 7.2 × 106; range, 6.1-8.4). The average number of leukophereses needed was 1.8 (mean, range 1.6-2.0). Colony growth was normal with 178.9 × 105 and 214.8 × 105 colonies counted in CFU-GM and BFU-E assays, respectively. Patients engrafted platelets and neutrophils on day 10 following transplantation. In conclusion, PBSC mobilization with paclitaxel and rhG-CSF results in a large number of mononuclear cells and CD34+ cells with normal clonogenic potential. The cells engraft normally following high-dose chemotherapy and autologous stem cell transplantation in high-risk breast cancer patients. These results demonstrate that paclitaxel with rhG-CSF is an efficient mobilizing agent in high-risk breast cancer patients.

Original languageEnglish (US)
Pages (from-to)415-421
Number of pages7
JournalJournal of Hematotherapy and Stem Cell Research
Volume11
Issue number2
DOIs
StatePublished - Apr 25 2002

ASJC Scopus subject areas

  • Immunology
  • Hematology

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    Meehan, K. R., Slack, R., Gehan, E., Herscowitz, H. B., Areman, E. M., Ebadi, M., Cairo, M. S., & Lippman, M. E. (2002). Mobilization of peripheral blood stem cells with paclitaxel and rhG-CSF in high-risk breast cancer patients. Journal of Hematotherapy and Stem Cell Research, 11(2), 415-421. https://doi.org/10.1089/152581602753658600