Mob2p interacts with the protein kinase Orb6p to promote coordination of cell polarity with cell cycle progression

Ming Chin Hou, David J. Wiley, Fulvia Verde, Dannel McCollum

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The molecular mechanisms that temporally and spatially coordinate cell morphogenesis with the cell cycle remain poorly understood. Here we describe the characterization of fission yeast Mob2p, a novel protein required for regulating cell polarity and cell cycle control. Deletion of mob2 is lethal and causes cells to become spherical, with depolarized actin and microtubule cytoskeletons. A decrease in Mob2p protein level results in a defect in the activation of bipolar growth. This phenotype is identical to that of mutants defective in the orb6 protein kinase gene, and we find that Mob2p physically interacts with Orb6p. In addition, overexpression of Mob2p, like that of Orb6p, results in a delay in the onset of mitosis. Mob2p localizes to the cell periphery and cytoplasm throughout the cell cycle and to the division site during late anaphase and telophase. Mob2p is unable to localize to the cell middle in mutants defective in actomyosin ring and septum formation. Our results suggest that Mob2p, along with Orb6p, is required for coordinating polarized cell growth during interphase with the onset of mitosis.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalJournal of Cell Science
Volume116
Issue number1
DOIs
StatePublished - Jan 1 2003

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Cell Polarity
Protein Kinases
Cell Cycle
Mitosis
Telophase
Actomyosin
Anaphase
Schizosaccharomyces
Interphase
Growth
Cell Cycle Checkpoints
Actin Cytoskeleton
Morphogenesis
Microtubules
Cytoplasm
Proteins
Phenotype
Genes

Keywords

  • Cell cycle
  • Cell polarity
  • Mob1p
  • Orb6p
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mob2p interacts with the protein kinase Orb6p to promote coordination of cell polarity with cell cycle progression. / Hou, Ming Chin; Wiley, David J.; Verde, Fulvia; McCollum, Dannel.

In: Journal of Cell Science, Vol. 116, No. 1, 01.01.2003, p. 125-135.

Research output: Contribution to journalArticle

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