Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells

Herman S Cheung, M. T. Story, D. J. McCarty

Research output: Contribution to journalArticle

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Abstract

Synthetic hydroxyapatite (HA) crystals in 1% serum stimulated 3H thymidine uptake into quiescent canine synovial fibroblasts and human foreskin fibroblast cultures, as did 10% serum. The onset of stimulation and peak uptake of thymidine after crystal addition were delayed by 2-3 hours as compared with the effects produced by 10% serum. Stimulation of 3H thymidine uptake was proportional to the serum concentration used. HA crystals (50 μg/ml) stimulated nuclide uptake at each serum concentration used. 3H thymidine uptake was also proportional to the dose of HA or calcium pyrophosphate dihydrate crystals, although larger doses of the latter crystal were required to produce equivalent effects. Not all particulates were effective mitogenic agents. Latex beads and diamond crystals had no effect. Monosodium urate crystals modestly stimulated and calcium urate crystals markedly stimulated nuclide uptake. The more complex crystals found in a naturally occurring condition (calcinosis) were as mitogenic as the pure synthetic HA. The synovial cell hyperplasia sometimes associated with crystals might be explained in part by their mitogenic activity.

Original languageEnglish
Pages (from-to)668-674
Number of pages7
JournalArthritis and Rheumatism
Volume27
Issue number6
StatePublished - Jan 1 1984
Externally publishedYes

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Calcium Pyrophosphate
Durapatite
Cultured Cells
Thymidine
Serum
Uric Acid
Fibroblasts
Foreskin
Calcinosis
Diamond
Microspheres
Hyperplasia
Canidae
Calcium

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Mitogenic effects of hydroxyapatite and calcium pyrophosphate dihydrate crystals on cultured mammalian cells. / Cheung, Herman S; Story, M. T.; McCarty, D. J.

In: Arthritis and Rheumatism, Vol. 27, No. 6, 01.01.1984, p. 668-674.

Research output: Contribution to journalArticle

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