Mitogenesis induced by calcium-containing crystals. Role of intracellular dissolution

Herman S. Cheung, Daniel J. McCarty

Research output: Contribution to journalArticle

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Abstract

Hydroxyapatite, like other calcium-containing crystals previously studied by us, is mitogenic for cultured human fibroblasts. This mitogenic effect is not a result of increased ambient calcium concentration due to extracellular crystal dissolution. Synthetic crystals labelled uniformly with calcium 45 (45Ca) undergo endocytosis when incubated with cells and are solubilized. Such solubilization is inhibited by chloroquine or ammonium chloride in concentrations that significantly block the mitogenic effect of crystals but not that of serum. The data suggest that mitogenesis and intracellular crystal dissolution are related phenomena.

Original languageEnglish (US)
Pages (from-to)63-70
Number of pages8
JournalExperimental Cell Research
Volume157
Issue number1
DOIs
StatePublished - Mar 1985
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology

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