Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits

Raquel Fonseca Guedes-Monteiro, José Ribamar Ferreira-Junior, Lucas Bleicher, Francisco G. Nóbrega, Antonio Barrientos, Mario H. Barros

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Saccharomyces cerevisiae mitoribosomes are specialized in the translation of a few number of highly hydrophobic membrane proteins, components of the oxidative phosphorylation system. Mitochondrial characteristics, such as the membrane system and its redox state driven mitoribosomes evolution through great diversion from their bacterial and cytosolic counterparts. Therefore, mitoribosome presents a considerable number of mitochondrial-specific proteins, as well as new protein extensions. In this work we characterize temperature sensitive mutants of the subunit bL34 present in the 54S large subunit. Although bL34 has bacterial homologs, in yeast it has a long 65 aminoacids mitochondrial N-terminal addressing sequence, here we demonstrate that it can be replaced by the mitochondrial addressing sequence of Neurospora crassa ATP9 gene. The bL34 temperature sensitive mutants present lowered translation of mitochondrial COX1 and COX3, which resulted in reduced cytochrome c oxidase activity and respiratory growth deficiency. The sedimentation properties of bL34 in sucrose gradients suggest that similarly to its bacterial homolog, bL34 is also a later participant in the process of mitoribosome biogenesis.

Original languageEnglish (US)
Pages (from-to)630-642
Number of pages13
JournalCell Biology International
Volume42
Issue number6
DOIs
StatePublished - Jun 1 2018

Keywords

  • cytochrome c oxidase
  • mitoribosome
  • mtDNA
  • respiratory chain
  • translation
  • yeast

ASJC Scopus subject areas

  • Cell Biology

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    Guedes-Monteiro, R. F., Ferreira-Junior, J. R., Bleicher, L., Nóbrega, F. G., Barrientos, A., & Barros, M. H. (2018). Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits. Cell Biology International, 42(6), 630-642. https://doi.org/10.1002/cbin.10913