Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits

Raquel Fonseca Guedes-Monteiro, José Ribamar Ferreira-Junior, Lucas Bleicher, Francisco G. Nóbrega, Antonio Barrientos, Mario H. Barros

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Saccharomyces cerevisiae mitoribosomes are specialized in the translation of a few number of highly hydrophobic membrane proteins, components of the oxidative phosphorylation system. Mitochondrial characteristics, such as the membrane system and its redox state driven mitoribosomes evolution through great diversion from their bacterial and cytosolic counterparts. Therefore, mitoribosome presents a considerable number of mitochondrial-specific proteins, as well as new protein extensions. In this work we characterize temperature sensitive mutants of the subunit bL34 present in the 54S large subunit. Although bL34 has bacterial homologs, in yeast it has a long 65 aminoacids mitochondrial N-terminal addressing sequence, here we demonstrate that it can be replaced by the mitochondrial addressing sequence of Neurospora crassa ATP9 gene. The bL34 temperature sensitive mutants present lowered translation of mitochondrial COX1 and COX3, which resulted in reduced cytochrome c oxidase activity and respiratory growth deficiency. The sedimentation properties of bL34 in sucrose gradients suggest that similarly to its bacterial homolog, bL34 is also a later participant in the process of mitoribosome biogenesis.

Original languageEnglish (US)
Pages (from-to)630-642
Number of pages13
JournalCell Biology International
Volume42
Issue number6
DOIs
StatePublished - Jun 1 2018

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Electron Transport Complex IV
Neurospora crassa
Temperature
Mitochondrial Proteins
Oxidative Phosphorylation
Oxidation-Reduction
Saccharomyces cerevisiae
Sucrose
Membrane Proteins
Yeasts
Membranes
Mitochondrial Ribosomes
Growth
Genes
Proteins

Keywords

  • cytochrome c oxidase
  • mitoribosome
  • mtDNA
  • respiratory chain
  • translation
  • yeast

ASJC Scopus subject areas

  • Cell Biology

Cite this

Guedes-Monteiro, R. F., Ferreira-Junior, J. R., Bleicher, L., Nóbrega, F. G., Barrientos, A., & Barros, M. H. (2018). Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits. Cell Biology International, 42(6), 630-642. https://doi.org/10.1002/cbin.10913

Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits. / Guedes-Monteiro, Raquel Fonseca; Ferreira-Junior, José Ribamar; Bleicher, Lucas; Nóbrega, Francisco G.; Barrientos, Antonio; Barros, Mario H.

In: Cell Biology International, Vol. 42, No. 6, 01.06.2018, p. 630-642.

Research output: Contribution to journalArticle

Guedes-Monteiro, RF, Ferreira-Junior, JR, Bleicher, L, Nóbrega, FG, Barrientos, A & Barros, MH 2018, 'Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits', Cell Biology International, vol. 42, no. 6, pp. 630-642. https://doi.org/10.1002/cbin.10913
Guedes-Monteiro, Raquel Fonseca ; Ferreira-Junior, José Ribamar ; Bleicher, Lucas ; Nóbrega, Francisco G. ; Barrientos, Antonio ; Barros, Mario H. / Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits. In: Cell Biology International. 2018 ; Vol. 42, No. 6. pp. 630-642.
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