Abstract
Objective: To study the mitochondrial respiratory chain enzyme activities in patients with idiopathic dilated cardiomyopathy (IDC). Methods: Mitochondrial respiratory chain enzyme activities were assessed spectrophotometrically in left ventricular tissue of 17 patients with IDC undergoing cardiac transplantation, as well as in two groups of controls: a group of six patients suffering from ischemic dilated cardiomyopathy (IC) also undergoing cardiac transplantation, and a group of 17 organ donors considered normal from a cardiac point of view. Cytochrome b gene from three IDC patients whose complex III activity was particularly low and from three controls was also sequenced. Results: We found that complex III enzymatic activity was lower not only in IDC but also in IC patients when compared with normal controls. When analysing cytochrome b gene we only found neutral polymorphisms previously described. Conclusions: In view of such results, we believe that the decrease of respiratory chain complex III activity found in some cases of IDC is a secondary phenomenon, and not due to a primary mitochondrial disease. (C) 2000 Elsevier Science B.V.
Original language | English (US) |
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Pages (from-to) | 860-865 |
Number of pages | 6 |
Journal | Cardiovascular Research |
Volume | 45 |
Issue number | 4 |
DOIs | |
State | Published - Mar 2000 |
Keywords
- Cardiomyopathy
- Energy metabolism
- Gene therapy
- Mitochondria
- Oxidative phosphorylation
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine