Mitochondrial function in heart muscle from patients with idiopathic dilated cardiomyopathy

Diana Jarreta, Josefina Orús, Antoni Barrientos, Oscar Miró, Eulàlia Roig, Magdalena Heras, Carlos T. Moraes, Francesc Cardellach, Jordi Casademont

Research output: Contribution to journalArticle

146 Scopus citations

Abstract

Objective: To study the mitochondrial respiratory chain enzyme activities in patients with idiopathic dilated cardiomyopathy (IDC). Methods: Mitochondrial respiratory chain enzyme activities were assessed spectrophotometrically in left ventricular tissue of 17 patients with IDC undergoing cardiac transplantation, as well as in two groups of controls: a group of six patients suffering from ischemic dilated cardiomyopathy (IC) also undergoing cardiac transplantation, and a group of 17 organ donors considered normal from a cardiac point of view. Cytochrome b gene from three IDC patients whose complex III activity was particularly low and from three controls was also sequenced. Results: We found that complex III enzymatic activity was lower not only in IDC but also in IC patients when compared with normal controls. When analysing cytochrome b gene we only found neutral polymorphisms previously described. Conclusions: In view of such results, we believe that the decrease of respiratory chain complex III activity found in some cases of IDC is a secondary phenomenon, and not due to a primary mitochondrial disease. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)860-865
Number of pages6
JournalCardiovascular Research
Volume45
Issue number4
DOIs
StatePublished - Mar 1 2000

Keywords

  • Cardiomyopathy
  • Energy metabolism
  • Gene therapy
  • Mitochondria
  • Oxidative phosphorylation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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