Misexpression of chick Vg1 in the marginal zone induces primitive streak formation

Shailan B. Shah, Isaac Skromne, Clifford R. Hume, Daniel S. Kessler, Kevin J. Lee, Claudio D. Stern, Jane Dodd

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

In the chick embryo, the primitive streak is the first axial structure to develop. The initiation of primitive streak formation in the posterior area pellucida is influenced by the adjacent posterior marginal zone (PMZ). We show here that chick Vg1 (cVg1) a member of the TGFβ family of signalling molecules whose homolog in Xenopus is implicated in mesoderm induction, is expressed in the PMZ of prestreak embryos. Ectopic expression of cVg1 protein in the marginal zone chick blastoderms directs the formation of a secondary primitive streak, which subsequently develops into an ectopic embryo. We have used cell marking techniques to show that cells that contribute to the ectopic primitive streak change fate, acquiring two distinct properties of primitive streak cells, defined by gene expression and cell movements. Furthermore, naive epiblast explants exposed to cVg1 protein in vitro acquire axial mesodermal properties. Together, these results show that cVg1 can mediate ectopic axis formation in the chick by inducing new cell fates and they permit the analysis of distinct events that occur during primitive streak formation.

Original languageEnglish (US)
Pages (from-to)5127-5138
Number of pages12
JournalDevelopment
Volume124
Issue number24
StatePublished - Dec 1 1997

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Keywords

  • Axis information
  • Chick
  • cVg1
  • Induction
  • Primitive streak
  • TGFβ
  • Vg1

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Shah, S. B., Skromne, I., Hume, C. R., Kessler, D. S., Lee, K. J., Stern, C. D., & Dodd, J. (1997). Misexpression of chick Vg1 in the marginal zone induces primitive streak formation. Development, 124(24), 5127-5138.