Misexpression of chick Vg1 in the marginal zone induces primitive streak formation

Shailan B. Shah, Isaac Skromne, Clifford R. Hume, Daniel S. Kessler, Kevin J. Lee, Claudio D. Stern, Jane Dodd

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

In the chick embryo, the primitive streak is the first axial structure to develop. The initiation of primitive streak formation in the posterior area pellucida is influenced by the adjacent posterior marginal zone (PMZ). We show here that chick Vg1 (cVg1) a member of the TGFβ family of signalling molecules whose homolog in Xenopus is implicated in mesoderm induction, is expressed in the PMZ of prestreak embryos. Ectopic expression of cVg1 protein in the marginal zone chick blastoderms directs the formation of a secondary primitive streak, which subsequently develops into an ectopic embryo. We have used cell marking techniques to show that cells that contribute to the ectopic primitive streak change fate, acquiring two distinct properties of primitive streak cells, defined by gene expression and cell movements. Furthermore, naive epiblast explants exposed to cVg1 protein in vitro acquire axial mesodermal properties. Together, these results show that cVg1 can mediate ectopic axis formation in the chick by inducing new cell fates and they permit the analysis of distinct events that occur during primitive streak formation.

Original languageEnglish
Pages (from-to)5127-5138
Number of pages12
JournalDevelopment
Volume124
Issue number24
StatePublished - Dec 1 1997
Externally publishedYes

Fingerprint

Primitive Streak
Embryonic Structures
Blastoderm
Germ Layers
Mesoderm
Chick Embryo
Xenopus
Cell Movement
Proteins
Gene Expression

Keywords

  • Axis information
  • Chick
  • cVg1
  • Induction
  • Primitive streak
  • TGFβ
  • Vg1

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Shah, S. B., Skromne, I., Hume, C. R., Kessler, D. S., Lee, K. J., Stern, C. D., & Dodd, J. (1997). Misexpression of chick Vg1 in the marginal zone induces primitive streak formation. Development, 124(24), 5127-5138.

Misexpression of chick Vg1 in the marginal zone induces primitive streak formation. / Shah, Shailan B.; Skromne, Isaac; Hume, Clifford R.; Kessler, Daniel S.; Lee, Kevin J.; Stern, Claudio D.; Dodd, Jane.

In: Development, Vol. 124, No. 24, 01.12.1997, p. 5127-5138.

Research output: Contribution to journalArticle

Shah, SB, Skromne, I, Hume, CR, Kessler, DS, Lee, KJ, Stern, CD & Dodd, J 1997, 'Misexpression of chick Vg1 in the marginal zone induces primitive streak formation', Development, vol. 124, no. 24, pp. 5127-5138.
Shah SB, Skromne I, Hume CR, Kessler DS, Lee KJ, Stern CD et al. Misexpression of chick Vg1 in the marginal zone induces primitive streak formation. Development. 1997 Dec 1;124(24):5127-5138.
Shah, Shailan B. ; Skromne, Isaac ; Hume, Clifford R. ; Kessler, Daniel S. ; Lee, Kevin J. ; Stern, Claudio D. ; Dodd, Jane. / Misexpression of chick Vg1 in the marginal zone induces primitive streak formation. In: Development. 1997 ; Vol. 124, No. 24. pp. 5127-5138.
@article{862969d2245e407da4d2cec425aa686b,
title = "Misexpression of chick Vg1 in the marginal zone induces primitive streak formation",
abstract = "In the chick embryo, the primitive streak is the first axial structure to develop. The initiation of primitive streak formation in the posterior area pellucida is influenced by the adjacent posterior marginal zone (PMZ). We show here that chick Vg1 (cVg1) a member of the TGFβ family of signalling molecules whose homolog in Xenopus is implicated in mesoderm induction, is expressed in the PMZ of prestreak embryos. Ectopic expression of cVg1 protein in the marginal zone chick blastoderms directs the formation of a secondary primitive streak, which subsequently develops into an ectopic embryo. We have used cell marking techniques to show that cells that contribute to the ectopic primitive streak change fate, acquiring two distinct properties of primitive streak cells, defined by gene expression and cell movements. Furthermore, naive epiblast explants exposed to cVg1 protein in vitro acquire axial mesodermal properties. Together, these results show that cVg1 can mediate ectopic axis formation in the chick by inducing new cell fates and they permit the analysis of distinct events that occur during primitive streak formation.",
keywords = "Axis information, Chick, cVg1, Induction, Primitive streak, TGFβ, Vg1",
author = "Shah, {Shailan B.} and Isaac Skromne and Hume, {Clifford R.} and Kessler, {Daniel S.} and Lee, {Kevin J.} and Stern, {Claudio D.} and Jane Dodd",
year = "1997",
month = "12",
day = "1",
language = "English",
volume = "124",
pages = "5127--5138",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "24",

}

TY - JOUR

T1 - Misexpression of chick Vg1 in the marginal zone induces primitive streak formation

AU - Shah, Shailan B.

AU - Skromne, Isaac

AU - Hume, Clifford R.

AU - Kessler, Daniel S.

AU - Lee, Kevin J.

AU - Stern, Claudio D.

AU - Dodd, Jane

PY - 1997/12/1

Y1 - 1997/12/1

N2 - In the chick embryo, the primitive streak is the first axial structure to develop. The initiation of primitive streak formation in the posterior area pellucida is influenced by the adjacent posterior marginal zone (PMZ). We show here that chick Vg1 (cVg1) a member of the TGFβ family of signalling molecules whose homolog in Xenopus is implicated in mesoderm induction, is expressed in the PMZ of prestreak embryos. Ectopic expression of cVg1 protein in the marginal zone chick blastoderms directs the formation of a secondary primitive streak, which subsequently develops into an ectopic embryo. We have used cell marking techniques to show that cells that contribute to the ectopic primitive streak change fate, acquiring two distinct properties of primitive streak cells, defined by gene expression and cell movements. Furthermore, naive epiblast explants exposed to cVg1 protein in vitro acquire axial mesodermal properties. Together, these results show that cVg1 can mediate ectopic axis formation in the chick by inducing new cell fates and they permit the analysis of distinct events that occur during primitive streak formation.

AB - In the chick embryo, the primitive streak is the first axial structure to develop. The initiation of primitive streak formation in the posterior area pellucida is influenced by the adjacent posterior marginal zone (PMZ). We show here that chick Vg1 (cVg1) a member of the TGFβ family of signalling molecules whose homolog in Xenopus is implicated in mesoderm induction, is expressed in the PMZ of prestreak embryos. Ectopic expression of cVg1 protein in the marginal zone chick blastoderms directs the formation of a secondary primitive streak, which subsequently develops into an ectopic embryo. We have used cell marking techniques to show that cells that contribute to the ectopic primitive streak change fate, acquiring two distinct properties of primitive streak cells, defined by gene expression and cell movements. Furthermore, naive epiblast explants exposed to cVg1 protein in vitro acquire axial mesodermal properties. Together, these results show that cVg1 can mediate ectopic axis formation in the chick by inducing new cell fates and they permit the analysis of distinct events that occur during primitive streak formation.

KW - Axis information

KW - Chick

KW - cVg1

KW - Induction

KW - Primitive streak

KW - TGFβ

KW - Vg1

UR - http://www.scopus.com/inward/record.url?scp=0031440022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031440022&partnerID=8YFLogxK

M3 - Article

C2 - 9362470

AN - SCOPUS:0031440022

VL - 124

SP - 5127

EP - 5138

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 24

ER -