MiR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFκB1

Himanshu Arora, Rehana Qureshi, Shunzi Jin, Ae Kyoung Park, Woong Yang Park

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

The activation of nuclear factor-kappa B1 (NFκB1) in cancer cells may confer resistance to ionizing radiation (IR). To enhance the therapeutic efficiency of IR in lung cancer, we screened for microRNAs (miRNAs) that suppress NFκB1 and observed their effects on ra-diosensitivity in a human lung cancer cell line. From time series data of miRNA expression in γ-irradiated H1299 human lung cancer cells, we found that the expression of miR-9 was inversely correlated with that of NFκB1. Overexpression of miR-9 down-regulated the level of NFκB1 in H1299 cells, and the surviving fraction of γ-irradiated cells was decreased. Interestingly, let-7g also suppressed the expression of NFκB1, although there was no canonical target site for let-7g in the NFκB1 3' untranslated region. From these results, we conclude that the expression of miR-9 and let-7g could enhance the efficiency of radiotherapy for lung cancer treatment through the inhibition of NFκB1.

Original languageEnglish (US)
Pages (from-to)298-304
Number of pages7
JournalExperimental and Molecular Medicine
Volume43
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

Keywords

  • Ionizing radiation
  • Let-7g
  • Lung cancer
  • MiR-9
  • NFκB1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'MiR-9 and let-7g enhance the sensitivity to ionizing radiation by suppression of NFκB1'. Together they form a unique fingerprint.

Cite this