Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Wei Zhang; Lena Lilja; Christina Bark; Per Olof Berggren; Björn Meister

doi:10.1016/j.bbrc.2004.05.208

Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Wei Zhang, Lena Lilja, Christina Bark, Per Olof Berggren, Björn Meister

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12 Scopus citations

Abstract

Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

Original language	English (US)
Pages (from-to)	717-721
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	320
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2004.05.208
State	Published - Jul 30 2004

Keywords

Exocytosis
Secretion
SNARE
Syntaxin

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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abstract = "Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.",

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AU - Bark, Christina

AU - Berggren, Per Olof

AU - Meister, Björn

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KW - Secretion

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