Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Wei Zhang, Lena Lilja, Christina Bark, Per Olof Berggren, Björn Meister

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

Original languageEnglish (US)
Pages (from-to)717-721
Number of pages5
JournalBiochemical and biophysical research communications
Volume320
Issue number3
DOIs
StatePublished - Jul 30 2004

Keywords

  • Exocytosis
  • Secretion
  • SNARE
  • Syntaxin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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