Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Wei Zhang; Lena Lilja; Christina Bark; Per Olof Berggren; Björn Meister

doi:10.1016/j.bbrc.2004.05.208

Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Wei Zhang, Lena Lilja, Christina Bark, Per Olof Berggren, Björn Meister

Surgery

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

Original language	English (US)
Pages (from-to)	717-721
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	320
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2004.05.208
State	Published - Jul 30 2004

Keywords

Exocytosis
Secretion
SNARE
Syntaxin

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/j.bbrc.2004.05.208

Fingerprint Dive into the research topics of 'Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells'. Together they form a unique fingerprint.

Cite this

@article{b964bffa08754cb48bbc9c945430c574,

title = "Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells",

abstract = "Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.",

keywords = "Exocytosis, Secretion, SNARE, Syntaxin",

author = "Wei Zhang and Lena Lilja and Christina Bark and Berggren, {Per Olof} and Bj{\"o}rn Meister",

note = "Funding Information: This work was supported by the Swedish Research Council (72X-10358 and 19X-00034), Swedish Diabetes Foundation, the Family Persson Foundation, Berth von Kantzow{\textquoteright}s Foundation, Novo Nordisk Foundation, {\AA}hl{\'e}n-stiftelsen, and Funds from Karolinska Institutet. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2004",

month = jul,

day = "30",

doi = "10.1016/j.bbrc.2004.05.208",

language = "English (US)",

volume = "320",

pages = "717--721",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

AU - Zhang, Wei

AU - Lilja, Lena

AU - Bark, Christina

AU - Berggren, Per Olof

AU - Meister, Björn

N1 - Funding Information: This work was supported by the Swedish Research Council (72X-10358 and 19X-00034), Swedish Diabetes Foundation, the Family Persson Foundation, Berth von Kantzow’s Foundation, Novo Nordisk Foundation, Åhlén-stiftelsen, and Funds from Karolinska Institutet. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/7/30

Y1 - 2004/7/30

N2 - Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

AB - Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

KW - Exocytosis

KW - Secretion

KW - SNARE

KW - Syntaxin

UR - http://www.scopus.com/inward/record.url?scp=3042724662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3042724662&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2004.05.208

DO - 10.1016/j.bbrc.2004.05.208

M3 - Article

C2 - 15240107

AN - SCOPUS:3042724662

VL - 320

SP - 717

EP - 721

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -

Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this