Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Wei Zhang, Lena Lilja, Christina Bark, Per Olof Berggren, Björn Meister

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

Original languageEnglish
Pages (from-to)717-721
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume320
Issue number3
DOIs
StatePublished - Jul 30 2004
Externally publishedYes

Fingerprint

Munc18 Proteins
Insulin-Secreting Cells
Islets of Langerhans
Exocytosis
Insulin
Rats
Proteins
Synaptic Vesicles
Cricetinae
Western Blotting
Cells
Cell Line
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Exocytosis
  • Secretion
  • SNARE
  • Syntaxin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells. / Zhang, Wei; Lilja, Lena; Bark, Christina; Berggren, Per Olof; Meister, Björn.

In: Biochemical and Biophysical Research Communications, Vol. 320, No. 3, 30.07.2004, p. 717-721.

Research output: Contribution to journalArticle

Zhang, Wei ; Lilja, Lena ; Bark, Christina ; Berggren, Per Olof ; Meister, Björn. / Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 320, No. 3. pp. 717-721.
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