Abstract
Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.
| Original language | English |
|---|---|
| Pages (from-to) | 717-721 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 320 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 30 2004 |
| Externally published | Yes |
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Keywords
- Exocytosis
- Secretion
- SNARE
- Syntaxin
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells. / Zhang, Wei; Lilja, Lena; Bark, Christina; Berggren, Per Olof; Meister, Björn.
In: Biochemical and Biophysical Research Communications, Vol. 320, No. 3, 30.07.2004, p. 717-721.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells
AU - Zhang, Wei
AU - Lilja, Lena
AU - Bark, Christina
AU - Berggren, Per Olof
AU - Meister, Björn
PY - 2004/7/30
Y1 - 2004/7/30
N2 - Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.
AB - Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.
KW - Exocytosis
KW - Secretion
KW - SNARE
KW - Syntaxin
UR - http://www.scopus.com/inward/record.url?scp=3042724662&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3042724662&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.05.208
DO - 10.1016/j.bbrc.2004.05.208
M3 - Article
C2 - 15240107
AN - SCOPUS:3042724662
VL - 320
SP - 717
EP - 721
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -