Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Wei Zhang; Lena Lilja; Christina Bark; Per Olof Berggren; Björn Meister

doi:10.1016/j.bbrc.2004.05.208

Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Wei Zhang, Lena Lilja, Christina Bark, Per Olof Berggren, Björn Meister

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Abstract

Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

Original language	English (US)
Pages (from-to)	717-721
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	320
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2004.05.208
State	Published - Jul 30 2004

Keywords

Exocytosis
Secretion
SNARE
Syntaxin

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/j.bbrc.2004.05.208

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title = "Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells",

abstract = "Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.",

keywords = "Exocytosis, Secretion, SNARE, Syntaxin",

author = "Wei Zhang and Lena Lilja and Christina Bark and Berggren, {Per Olof} and Bj{\"o}rn Meister",

note = "Funding Information: This work was supported by the Swedish Research Council (72X-10358 and 19X-00034), Swedish Diabetes Foundation, the Family Persson Foundation, Berth von Kantzow{\textquoteright}s Foundation, Novo Nordisk Foundation, {\AA}hl{\'e}n-stiftelsen, and Funds from Karolinska Institutet. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2004",

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doi = "10.1016/j.bbrc.2004.05.208",

language = "English (US)",

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T1 - Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

AU - Zhang, Wei

AU - Lilja, Lena

AU - Bark, Christina

AU - Berggren, Per Olof

AU - Meister, Björn

N1 - Funding Information: This work was supported by the Swedish Research Council (72X-10358 and 19X-00034), Swedish Diabetes Foundation, the Family Persson Foundation, Berth von Kantzow’s Foundation, Novo Nordisk Foundation, Åhlén-stiftelsen, and Funds from Karolinska Institutet. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/7/30

Y1 - 2004/7/30

N2 - Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

AB - Munc-18-interacting (Mint) proteins are adaptors involved in regulation of synaptic vesicle exocytosis. We have investigated expression and cellular localization of Mint1 in pancreatic islets with special reference to insulin-secreting β-cells. Western blotting showed that Mint1 was expressed in hamster (HIT-T15) and rat (RINm5F) β-cell lines. Mint1 immunoreactivity was preferentially localized to the periphery of individual islet cells. RT-PCR analysis revealed that apart from Mint1, RINm5F cells and rat islets also transcribed the mRNAs for Mint2 and Mint3. Expression of Mint proteins in pancreatic β-cells suggests a functional role for these proteins in insulin granule exocytosis.

KW - Exocytosis

KW - Secretion

KW - SNARE

KW - Syntaxin

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ER -

Mint1, a Munc-18-interacting protein, is expressed in insulin-secreting β-cells

Abstract

Keywords

ASJC Scopus subject areas

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