Minisatellite mutation frequency in human sperm following radiotherapy

Celia A. May, Keiji Tamaki, Rita Neumann, Gene Wilson, Gunar Zagars, Alan Pollack, Yuri E. Dubrova, Alec J. Jeffreys, Marvin L. Meistrich

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Screening pedigrees for inherited minisatellite length changes provides an efficient means of monitoring repeat DNA instability but has given rise to apparently contradictory results regarding the effects of radiation on the human germline. To explore this further in individuals with known radiation doses and to potentially gain information on the timing of mutation induction, we have used an extremely sensitive single molecule approach to quantify the frequencies of mutation at the hypervariable minisatellites B6.7 and CEB1 in the sperm of three seminoma patients following hemipelvic radiotherapy. Scattered radiation doses to the testicles were monitored and pre-treatment sperm DNA was compared with sperm derived from irradiated pre- meiotic, meiotic and post-meiotic cells. We show no evidence for mutation induction in any of the patients and discuss this finding in the context of previous population studies using minisatellites as reporter systems, one of which provided evidence for radiation-induced germline mutation. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume453
Issue number1
DOIs
StatePublished - Sep 20 2000
Externally publishedYes

Keywords

  • Germline mutation
  • Ionizing radiation
  • Minisatellite
  • Radiotherapy
  • Sperm

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis

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  • Cite this

    May, C. A., Tamaki, K., Neumann, R., Wilson, G., Zagars, G., Pollack, A., Dubrova, Y. E., Jeffreys, A. J., & Meistrich, M. L. (2000). Minisatellite mutation frequency in human sperm following radiotherapy. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 453(1), 67-75. https://doi.org/10.1016/S0027-5107(00)00085-3