Migration of newly generated neurons upon ependymally derived radial guide cells in explant cultures of the adult songbird forebrain.

S. A. Goldman, Vance Lemmon, S. S. Chin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The adult songbird forebrain undergoes neuronal production throughout adulthood, with the production of new neurons in discrete regions of the neostriatal ventricular zone. Upon mitogenesis, these new neurons migrate into the subjacent brain parenchyma along radially directed guide fibers. In long-term ventricular zone explant cultures, derived from the higher vocal center of the adult canary, newly migratory neurons were found to associate preferentially with a characteristic substrate cell type. These small, parvonuclear substrate cells formed tightly packed epithelioid sheets, in which ciliated ependymal cells were common, as recognized by both live observation and electron microscopy. A subpopulation of these cells was immunostained by monoclonal antibody 3A7, which preferentially stains the guide fiber network of the adult avian brain. These 3A7+ cells included ependymal cells and bipolar radial cells, as well as morphologically defined astrocytes. As they matured in vitro, the 3A7+ bipolar radial cells extended long, unbranching fibers, which ultimately traversed the culture substrate. Like ependymal cells, they supported neuronal migration. These cells were likely homologous to radial guide cells in vivo. Thus, neuronal migration in adult avian forebrain culture occurred upon guide cells of ependymal derivation.

Original languageEnglish
Pages (from-to)150-160
Number of pages11
JournalGLIA
Volume8
Issue number3
StatePublished - Jul 1 1993
Externally publishedYes

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Songbirds
Prosencephalon
Neurons
High Vocal Center
Canaries
Brain
Astrocytes
Electron Microscopy
Coloring Agents
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Migration of newly generated neurons upon ependymally derived radial guide cells in explant cultures of the adult songbird forebrain. / Goldman, S. A.; Lemmon, Vance; Chin, S. S.

In: GLIA, Vol. 8, No. 3, 01.07.1993, p. 150-160.

Research output: Contribution to journalArticle

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