Microvessels Isolated from Rat Brain: Localization of Astrocyte Processes by Immunohistochemical Techniques

Fredric P. White, Gary R. Dutton, Michael D. Norenberg

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Microvascular networks were isolated from rat telencephalon by density gradient centrifugation. The vessels prepared were characterized morphologically at the light and electron microscope level and immunohistochemically by the localization of glutamine synthetase and glial fibrillary acidic protein, two proteins found almost exclusively in astrocytes. The vast majority of the vessels prepared contained more than just endothelial cells surrounded by a basement membrane. Many arterioles were found still retaining their smooth muscle cells. Pericytes were found in association with most of the venules and many of the capillaries. Astrocyte processes remained attached to most of the microvessels. These results show that vessels prepared from rat brain still maintain most of their complex intercellular contacts and must be viewed as a heterogeneous network of cells.

Original languageEnglish (US)
Pages (from-to)328-332
Number of pages5
JournalJournal of neurochemistry
Volume36
Issue number1
DOIs
StatePublished - Jan 1981
Externally publishedYes

Keywords

  • Astrocytes
  • Glial fibrillary acidic protein
  • Glutamine synthetase
  • Immunohistochemistry
  • Microvessels

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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