Abstract
The Schizosaccharomyces pombe mutant, ban5-4, displays aberrant mitochondrial distribution. Incubation of this conditional-lethal mutant at the nonpermissive temperature led to aggregated mitochondria that were distributed asymmetrically within the cell. Development of this mitochondrial asymmetry but not mitochondrial aggregation required progression through the cell division cycle. Genetic analysis revealed that ban5-4 is an allele of atb2 encoding α2-tubulin. Consistent with this finding, cells with the cold- sensitive nda3 mutation in β-tubulin displayed aggregated and asymmetrically distributed mitochondria after incubation at lowered temperatures. These results indicate that microtubules mediate mitochondrial distribution in fission yeast and provide the first genetic evidence for the role of microtubules in mitochondrial movement.
| Original language | English |
|---|---|
| Pages (from-to) | 11664-11668 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 93 |
| Issue number | 21 |
| DOIs | |
| State | Published - Oct 15 1996 |
| Externally published | Yes |
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Keywords
- cytoskeleton
- organelle movement
- Schizosaccharomyces pombe
ASJC Scopus subject areas
- Genetics
- General
Cite this
Microtubules mediate mitochondrial distribution in fission yeast. / Yaffe, Michael P.; Harata, Dai; Verde, Fulvia; Eddison, Mark; Toda, Takashi; Nurse, Paul.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 21, 15.10.1996, p. 11664-11668.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microtubules mediate mitochondrial distribution in fission yeast
AU - Yaffe, Michael P.
AU - Harata, Dai
AU - Verde, Fulvia
AU - Eddison, Mark
AU - Toda, Takashi
AU - Nurse, Paul
PY - 1996/10/15
Y1 - 1996/10/15
N2 - The Schizosaccharomyces pombe mutant, ban5-4, displays aberrant mitochondrial distribution. Incubation of this conditional-lethal mutant at the nonpermissive temperature led to aggregated mitochondria that were distributed asymmetrically within the cell. Development of this mitochondrial asymmetry but not mitochondrial aggregation required progression through the cell division cycle. Genetic analysis revealed that ban5-4 is an allele of atb2 encoding α2-tubulin. Consistent with this finding, cells with the cold- sensitive nda3 mutation in β-tubulin displayed aggregated and asymmetrically distributed mitochondria after incubation at lowered temperatures. These results indicate that microtubules mediate mitochondrial distribution in fission yeast and provide the first genetic evidence for the role of microtubules in mitochondrial movement.
AB - The Schizosaccharomyces pombe mutant, ban5-4, displays aberrant mitochondrial distribution. Incubation of this conditional-lethal mutant at the nonpermissive temperature led to aggregated mitochondria that were distributed asymmetrically within the cell. Development of this mitochondrial asymmetry but not mitochondrial aggregation required progression through the cell division cycle. Genetic analysis revealed that ban5-4 is an allele of atb2 encoding α2-tubulin. Consistent with this finding, cells with the cold- sensitive nda3 mutation in β-tubulin displayed aggregated and asymmetrically distributed mitochondria after incubation at lowered temperatures. These results indicate that microtubules mediate mitochondrial distribution in fission yeast and provide the first genetic evidence for the role of microtubules in mitochondrial movement.
KW - cytoskeleton
KW - organelle movement
KW - Schizosaccharomyces pombe
UR - http://www.scopus.com/inward/record.url?scp=0029957226&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029957226&partnerID=8YFLogxK
U2 - 10.1073/pnas.93.21.11664
DO - 10.1073/pnas.93.21.11664
M3 - Article
VL - 93
SP - 11664
EP - 11668
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 21
ER -