Microtubules mediate mitochondrial distribution in fission yeast

Michael P. Yaffe, Dai Harata, Fulvia Verde, Mark Eddison, Takashi Toda, Paul Nurse

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The Schizosaccharomyces pombe mutant, ban5-4, displays aberrant mitochondrial distribution. Incubation of this conditional-lethal mutant at the nonpermissive temperature led to aggregated mitochondria that were distributed asymmetrically within the cell. Development of this mitochondrial asymmetry but not mitochondrial aggregation required progression through the cell division cycle. Genetic analysis revealed that ban5-4 is an allele of atb2 encoding α2-tubulin. Consistent with this finding, cells with the cold- sensitive nda3 mutation in β-tubulin displayed aggregated and asymmetrically distributed mitochondria after incubation at lowered temperatures. These results indicate that microtubules mediate mitochondrial distribution in fission yeast and provide the first genetic evidence for the role of microtubules in mitochondrial movement.

Original languageEnglish
Pages (from-to)11664-11668
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number21
DOIs
StatePublished - Oct 15 1996
Externally publishedYes

Fingerprint

Schizosaccharomyces
Tubulin
Microtubules
Mitochondria
Temperature
Cell Cycle
Alleles
Mutation

Keywords

  • cytoskeleton
  • organelle movement
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Microtubules mediate mitochondrial distribution in fission yeast. / Yaffe, Michael P.; Harata, Dai; Verde, Fulvia; Eddison, Mark; Toda, Takashi; Nurse, Paul.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 21, 15.10.1996, p. 11664-11668.

Research output: Contribution to journalArticle

Yaffe, Michael P. ; Harata, Dai ; Verde, Fulvia ; Eddison, Mark ; Toda, Takashi ; Nurse, Paul. / Microtubules mediate mitochondrial distribution in fission yeast. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 21. pp. 11664-11668.
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