Microtubules in cone myoid elongation in the teleost retina

R. H. Warren, B. Burnside

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Abstract

The myoids of retinal cone cells of the blue-striped grunt (Haemulon sciurus) undergo significant elongation during dark adaptation of the retina. Longitudinally oriented microtubules are present in myoids both before and after elongation. Injection of colchicine into the vitreous of the eye in vivo disrupts the microtubules in the myoids and prevents dark-adaptive myoid elongation. Counts of microtubules in transverse sections along the lengths of elongating myoids show that there is a uniform decrease in the number of microtubules at any one point along the myoid as the myoid elongates. The magnitude of the decrease is proportional to the extent of the elongation. The product of the mean myoid microtubule number (determined from counts at progressive intervals along the myoid) and the myoid length remains essentially constant during myoid elongation, indicating that the total quantity of microtubules in the myoid does not increase with elongation. Serial section tracings of the microtubules along the myoids suggest that individual microtubules do not extend the length of the myoid and that the myoid microtubular apparatus consists of bundles of overlapping shorter microtubules. The authors propose that elongation of the myoid is accompanied by sliding redistribution of microtubules along the length of the myoid, and that the sliding may be generated by interactions between microtubules in regions where they closely overlap in bundles. The authors find no evidence for the involvement of discrete, electron-dense microtubular organizing centers in myoid elongation.

Original languageEnglish (US)
Pages (from-to)247-259
Number of pages13
JournalJournal of Cell Biology
Volume78
Issue number1
DOIs
StatePublished - Jan 1 1978

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ASJC Scopus subject areas

  • Cell Biology

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