Microtubules and actin in giant nerve fibers of the spiny lobster, Panulirus argus

Robert H. Warren, Robert W. Rubin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Giant axons of the spiny lobster, Panulirus argus, are filled with microtubules that are decorated with fine, irregular filaments. Mitochondria and membranelimited clear vesicles are the only other distinguishable elements in the axoplasm and are located around the periphery of the axon near the axolemma. Neither 100 Å neurofilaments nor 70 Å microfilaments are evident in fixed, intact axons or in negatively stained axoplasm. Actin-like microfilaments are a prominent constituent of the glial cells that closely ensheathe the axons, and gel electrophoresis studies suggest that most of the actin in the nerve fibers is located in the glia rather than in the axons. Studies of isolated axoplasm indicate that microtubules are the primary elements stabilizing the axoplasm. The microtubules in the isolated axoplasm are disrupted by Ca2+ in the medium in the presence of protease inhibitors.

Original languageEnglish
Pages (from-to)687-697
Number of pages11
JournalTissue and Cell
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1978
Externally publishedYes

Fingerprint

Palinuridae
Panulirus argus
nerve fibers
lobsters
Nerve Fibers
axons
Microtubules
microtubules
actin
Axons
Actins
microfilaments
Actin Cytoskeleton
Neuroglia
Intermediate Filaments
neuroglia
proteinase inhibitors
Protease Inhibitors
gel electrophoresis
Electrophoresis

ASJC Scopus subject areas

  • Insect Science
  • Cell Biology

Cite this

Microtubules and actin in giant nerve fibers of the spiny lobster, Panulirus argus. / Warren, Robert H.; Rubin, Robert W.

In: Tissue and Cell, Vol. 10, No. 4, 01.01.1978, p. 687-697.

Research output: Contribution to journalArticle

Warren, Robert H. ; Rubin, Robert W. / Microtubules and actin in giant nerve fibers of the spiny lobster, Panulirus argus. In: Tissue and Cell. 1978 ; Vol. 10, No. 4. pp. 687-697.
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