Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity

Pedro J Salas, D. E. Misek, D. E. Vega-Salas, D. Gundersen, M. Cereijido, E. Rodriguez-Boulan

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

We have studied the role of microtubules and actin filaments in the biogenesis of epithelial cell surface polarity, using influenza hemagglutinin and vesicular stomatitis G protein as model apical and basolateral proteins in infected Madin-Darby canine kidney cells. Addition of colchicine or nocodazole to confluent monolayers at concentrations sufficient to completely disassemble microtubules did not affect the asymmetric budding of influenza or vesicular stomatitis virus and only slightly reduced the typical asymmetric surface distribution of their envelope proteins, despite extensive cytoplasmic redistribution of the Golgi apparatus. Alteration of microtubular function by taxol or dissociation of actin filaments by cytochalasin D also failed to have a significant effect. Furthermore, neither colchicine nor cytochalasin D pretreatment blocked the ability of subconfluent Madin-Darby canine kidney cells to sustain polarized budding of influenza virus a few hours after attachment to the substrate. Our results indicate that domain-specific microtubule or actin filament 'tracks' are not responsible for the vectorial delivery of apically or basolaterally directed transport vesicles. In conjunction with currently available evidence, they are compatible with a model in which receptors in the cytoplasmic aspect of apical or basolateral regions provide vectoriality to the transport of vesicles carrying plasma membrane protein to their final surface localization.

Original languageEnglish
Pages (from-to)1853-1867
Number of pages15
JournalJournal of Cell Biology
Volume102
Issue number5
StatePublished - Aug 13 1986
Externally publishedYes

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Cell Polarity
Actin Cytoskeleton
Microtubules
Cytochalasin D
Transport Vesicles
Madin Darby Canine Kidney Cells
Epithelial Cells
Colchicine
Human Influenza
Nocodazole
Vesicular Stomatitis
Hemagglutinins
Golgi Apparatus
Cytoplasmic and Nuclear Receptors
Paclitaxel
Orthomyxoviridae
Blood Proteins
Membrane Proteins
Proteins
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

Cite this

Salas, P. J., Misek, D. E., Vega-Salas, D. E., Gundersen, D., Cereijido, M., & Rodriguez-Boulan, E. (1986). Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity. Journal of Cell Biology, 102(5), 1853-1867.

Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity. / Salas, Pedro J; Misek, D. E.; Vega-Salas, D. E.; Gundersen, D.; Cereijido, M.; Rodriguez-Boulan, E.

In: Journal of Cell Biology, Vol. 102, No. 5, 13.08.1986, p. 1853-1867.

Research output: Contribution to journalArticle

Salas, PJ, Misek, DE, Vega-Salas, DE, Gundersen, D, Cereijido, M & Rodriguez-Boulan, E 1986, 'Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity', Journal of Cell Biology, vol. 102, no. 5, pp. 1853-1867.
Salas PJ, Misek DE, Vega-Salas DE, Gundersen D, Cereijido M, Rodriguez-Boulan E. Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity. Journal of Cell Biology. 1986 Aug 13;102(5):1853-1867.
Salas, Pedro J ; Misek, D. E. ; Vega-Salas, D. E. ; Gundersen, D. ; Cereijido, M. ; Rodriguez-Boulan, E. / Microtubules and actin filaments are not critically involved in the biogenesis of epithelial cell surface polarity. In: Journal of Cell Biology. 1986 ; Vol. 102, No. 5. pp. 1853-1867.
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