Microtubule-associated protein 1a is involved in the early development of the rat spinal cord

Martin Oudega, F. Touri, M. G M Deenen, B. M. Riederer, E. Marani

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The expression of microtubule-associated protein 1a (MAP1a) in the developing rat spinal cord was studied using the monoclonal antibody BW6. Immunoblots of microtubule preparations revealed the presence of MAP1a in spinal cord tissue of rats aged embryonal day 16 and postnatal day 0. The spinal cord matrix layer, between embryonal days 12-17, displayed a pattern of MAP1a-positive processes, horizontally oriented in between the membrane limitans interna and externa. The mantle layer stained intensely for MAP1a between embryonal day 12 and postnatal day 2. MAP1a was found in neuronal cell bodies, axons and dendrites, located mainly in the ventral and intermediate mantle layer. In the marginal layer, MAP1a-positive axons could be observed between embryonal days 14-18. During further development, the intensity of the MAP1a staining in the spinal columns gradually decreased. These expression patterns indicate an involvement of MAP1a in the proliferation and differentiation of neuroblasts, and the maturation of the long spinal fiber sytems, i.e. early events in spinal cord development.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalNeuroscience Letters
Volume246
Issue number2
DOIs
StatePublished - Apr 24 1998

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Microtubule-Associated Proteins
Spinal Cord
Axons
Dendrites
Microtubules
Spine
Monoclonal Antibodies
Staining and Labeling
Membranes

Keywords

  • Central nervous system
  • Cytoskeleton
  • Microtubule- associated protein
  • Ontogeny
  • Subtype
  • Vimentin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Microtubule-associated protein 1a is involved in the early development of the rat spinal cord. / Oudega, Martin; Touri, F.; Deenen, M. G M; Riederer, B. M.; Marani, E.

In: Neuroscience Letters, Vol. 246, No. 2, 24.04.1998, p. 81-84.

Research output: Contribution to journalArticle

Oudega, Martin ; Touri, F. ; Deenen, M. G M ; Riederer, B. M. ; Marani, E. / Microtubule-associated protein 1a is involved in the early development of the rat spinal cord. In: Neuroscience Letters. 1998 ; Vol. 246, No. 2. pp. 81-84.
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