Microsurgical denervation of rat spermatic cord: Safety and efficacy data

Melissa A. Laudano, E. Charles Osterberg, Seema Sheth, Ranjith Ramasamy, Joshua Sterling, Sushmita Mukherjee, Brian D. Robinson, Sijo Parekattil, Marc Goldstein, Peter N. Schlegel, Philip S. Li

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective To describe a microsurgical technique for denervation of the spermatic cord and use of multiphoton microscopy (MPM) laser to identify and ablate residual nerves after microsurgical denervation. To evaluate structural and functional changes in the rat testis and vas deferens after denervation. Materials and Methods Nine Sprague-Dawley rats were divided into three experimental groups: sham, microsurgical denervation of the spermatic cord (MDSC), and MDSC immediately followed by laser ablation with MPM. At 2 months after surgery, we assessed testicular volume, functional circulation of the testicular artery with Doppler, patency of the vas deferens, and histology of the testis and vas deferens. Results There was a significant decrease in the median number of nerves remaining around the vas deferens with MDSC alone (3.5 nerves) or MDSC with MPM (1.5 nerves) compared with sham rats (15.5 nerves) (P = 0.003). Although, MDSC with MPM resulted in the fewest remaining nerves, this result was similar to MDSC alone (P = 0.29). No deleterious effects on spermatogenesis or vas patency were seen in the experimental groups when compared with the sham rats. Conclusion A microsurgical approach can be used to effectively and safely denervate the rat spermatic cord with minimal changes to structure and function of the testis and vas deferens. MPM can be used as an adjunct to identify and ablate residual nerves after MDSC.

Original languageEnglish (US)
Pages (from-to)795-800
Number of pages6
JournalBJU International
Volume113
Issue number5
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Spermatic Cord
Denervation
Safety
Vas Deferens
Microscopy
Testis
Median Nerve
Laser Therapy
Spermatogenesis
Confocal Microscopy
Sprague Dawley Rats
Histology
Arteries

Keywords

  • denervation
  • microsurgery
  • safety

ASJC Scopus subject areas

  • Urology

Cite this

Laudano, M. A., Osterberg, E. C., Sheth, S., Ramasamy, R., Sterling, J., Mukherjee, S., ... Li, P. S. (2014). Microsurgical denervation of rat spermatic cord: Safety and efficacy data. BJU International, 113(5), 795-800. https://doi.org/10.1111/bju.12421

Microsurgical denervation of rat spermatic cord : Safety and efficacy data. / Laudano, Melissa A.; Osterberg, E. Charles; Sheth, Seema; Ramasamy, Ranjith; Sterling, Joshua; Mukherjee, Sushmita; Robinson, Brian D.; Parekattil, Sijo; Goldstein, Marc; Schlegel, Peter N.; Li, Philip S.

In: BJU International, Vol. 113, No. 5, 2014, p. 795-800.

Research output: Contribution to journalArticle

Laudano, MA, Osterberg, EC, Sheth, S, Ramasamy, R, Sterling, J, Mukherjee, S, Robinson, BD, Parekattil, S, Goldstein, M, Schlegel, PN & Li, PS 2014, 'Microsurgical denervation of rat spermatic cord: Safety and efficacy data', BJU International, vol. 113, no. 5, pp. 795-800. https://doi.org/10.1111/bju.12421
Laudano MA, Osterberg EC, Sheth S, Ramasamy R, Sterling J, Mukherjee S et al. Microsurgical denervation of rat spermatic cord: Safety and efficacy data. BJU International. 2014;113(5):795-800. https://doi.org/10.1111/bju.12421
Laudano, Melissa A. ; Osterberg, E. Charles ; Sheth, Seema ; Ramasamy, Ranjith ; Sterling, Joshua ; Mukherjee, Sushmita ; Robinson, Brian D. ; Parekattil, Sijo ; Goldstein, Marc ; Schlegel, Peter N. ; Li, Philip S. / Microsurgical denervation of rat spermatic cord : Safety and efficacy data. In: BJU International. 2014 ; Vol. 113, No. 5. pp. 795-800.
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