TY - JOUR
T1 - Microscopic and histochemical manifestations of hyaline cartilage dynamics
AU - Malinin, George I.
AU - Malinin, Theodore I.
N1 - Funding Information:
The authors wish to thank EL GENDLER, M. D. for his continuous support and interest in this work. Support from the Mannheimer Foundation, and the Kucklinsky Foundation is also gratefully acknowledged. We wish to thank Ms. PAMELLA POLARD for histological sections, Mr. THOMAS BURKE for photography, Ms. JESSICA COLLADO for typing the manuscript and Ms. DOROTHY R. MALININ for proofreading the manuscript.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 1999
Y1 - 1999
N2 - Structure and function of hyaline cartilages has been the focus of many correlative studies for over a hundred years. Much of what is known regarding dynamics and function of cartilage constituents has been derived or inferred from biochemical and electron microscopic investigations. Here we show that in conjunction with ultrastructural, and high-magnification transmission light and polarization microscopy, the well-developed histochemical methods are indispensable for the analysis of cartilage dynamics. Microscopically demonstrable aspects of cartilage dynamics include, but are not limited to, formation of the intracellular liquid crystals, phase transitions of the extracellular matrix and tubular connections between chondrocytes. The role of the interchondrocytic liquid crystals is considered in terms of the tensegrity hypothesis and non-apoptotic cell death. Phase transitions of the extracellular matrix are discussed in terms of self-alignment of chondrons, matrix guidance pathways and cartilage growth in the absence of mitosis. The possible role of nonenzymatic glycation reactions in cartilage dynamics is also reviewed.
AB - Structure and function of hyaline cartilages has been the focus of many correlative studies for over a hundred years. Much of what is known regarding dynamics and function of cartilage constituents has been derived or inferred from biochemical and electron microscopic investigations. Here we show that in conjunction with ultrastructural, and high-magnification transmission light and polarization microscopy, the well-developed histochemical methods are indispensable for the analysis of cartilage dynamics. Microscopically demonstrable aspects of cartilage dynamics include, but are not limited to, formation of the intracellular liquid crystals, phase transitions of the extracellular matrix and tubular connections between chondrocytes. The role of the interchondrocytic liquid crystals is considered in terms of the tensegrity hypothesis and non-apoptotic cell death. Phase transitions of the extracellular matrix are discussed in terms of self-alignment of chondrons, matrix guidance pathways and cartilage growth in the absence of mitosis. The possible role of nonenzymatic glycation reactions in cartilage dynamics is also reviewed.
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U2 - 10.1016/s0079-6336(99)80006-0
DO - 10.1016/s0079-6336(99)80006-0
M3 - Article
C2 - 10672618
AN - SCOPUS:0033251410
VL - 34
SP - 163
EP - 239
JO - Progress in Histochemistry and Cytochemistry
JF - Progress in Histochemistry and Cytochemistry
SN - 0079-6336
IS - 3
ER -