Microsatellite primers for the Atlantic coastal killifish, Fundulus heteroclitus, with applicability to related Fundulus species

Stephanie M. Adams, Marjorie F Oleksiak, David D. Duvernell

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The mummichog (Fundulus heteroclitus), a common Atlantic coastal killifish, is a model vertebrate species for the study of molecular genetic variation in natural populations and of environmental toxicology. We report the development of a set of 20 microsatellite loci in this species. Average expected heterozygosity across all loci was 0.84 (range: 0.60-0.97), revealing a high level of variability at most loci. A survey of seven additional Fundulus species yielded one or two robust amplification products in over half (63%) of the species-primer combinations tested. Therefore, many of these loci will also prove useful in studies of other members of the genus Fundulus.

Original languageEnglish
Pages (from-to)275-277
Number of pages3
JournalMolecular Ecology Notes
Volume5
Issue number2
DOIs
StatePublished - Jun 1 2005
Externally publishedYes

Fingerprint

Fundulidae
Fundulus
Fundulus heteroclitus
Microsatellite Repeats
Amplification
microsatellite repeats
loci
ecotoxicology
Ecotoxicology
toxicology
molecular genetics
heterozygosity
genetic variation
amplification
vertebrate
vertebrates
Vertebrates
Molecular Biology
Population

Keywords

  • Fundulus catenatus
  • Fundulus cingulatus
  • Fundulus confluentus
  • Fundulus majalis
  • Fundulus olivaceus
  • Fundulus seminolis
  • Fundulus similis

ASJC Scopus subject areas

  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Microsatellite primers for the Atlantic coastal killifish, Fundulus heteroclitus, with applicability to related Fundulus species. / Adams, Stephanie M.; Oleksiak, Marjorie F; Duvernell, David D.

In: Molecular Ecology Notes, Vol. 5, No. 2, 01.06.2005, p. 275-277.

Research output: Contribution to journalArticle

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