MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi

Evan K. Maxwell, Joseph F. Ryan, Christine E. Schnitzler, William Browne, Andreas D. Baxevanis

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: MicroRNAs play a vital role in the regulation of gene expression and have been identified in every animal with a sequenced genome examined thus far, except for the placozoan Trichoplax. The genomic repertoires of metazoan microRNAs have become increasingly endorsed as phylogenetic characters and drivers of biological complexity.Results: In this study, we report the first investigation of microRNAs in a species from the phylum Ctenophora. We use short RNA sequencing and the assembled genome of the lobate ctenophore Mnemiopsis leidyi to show that this species appears to lack any recognizable microRNAs, as well as the nuclear proteins Drosha and Pasha, which are critical to canonical microRNA biogenesis. This finding represents the first reported case of a metazoan lacking a Drosha protein.Conclusions: Recent phylogenomic analyses suggest that Mnemiopsis may be the earliest branching metazoan lineage. If this is true, then the origins of canonical microRNA biogenesis and microRNA-mediated gene regulation may postdate the last common metazoan ancestor. Alternatively, canonical microRNA functionality may have been lost independently in the lineages leading to both Mnemiopsis and the placozoan Trichoplax, suggesting that microRNA functionality was not critical until much later in metazoan evolution.

Original languageEnglish
Article number714
JournalBMC Genomics
Volume13
Issue number1
DOIs
StatePublished - Dec 20 2012

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Ctenophora
MicroRNAs
Genome
Placozoa
RNA Sequence Analysis
Gene Expression Regulation
Nuclear Proteins

Keywords

  • Ctenophore
  • Drosha
  • Metazoa
  • Microprocessor complex
  • MicroRNA
  • MiRNA
  • Mnemiopsis leidyi
  • Pasha
  • Ribonuclease III
  • RNase III

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi. / Maxwell, Evan K.; Ryan, Joseph F.; Schnitzler, Christine E.; Browne, William; Baxevanis, Andreas D.

In: BMC Genomics, Vol. 13, No. 1, 714, 20.12.2012.

Research output: Contribution to journalArticle

Maxwell, Evan K. ; Ryan, Joseph F. ; Schnitzler, Christine E. ; Browne, William ; Baxevanis, Andreas D. / MicroRNAs and essential components of the microRNA processing machinery are not encoded in the genome of the ctenophore Mnemiopsis leidyi. In: BMC Genomics. 2012 ; Vol. 13, No. 1.
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