Abstract
The relatively new field of microRNA (miR) has experienced rapid growth in methodology associated with its detection and bioanalysis as well as with its role in -omics research, clinical diagnostics, and new therapeutic strategies. The breadth of this area of research and the seemingly exponential increase in number of publications on the subject can present scientists new to the field with a daunting amount of information to evaluate. This review aims to provide a collective overview of miR detection methods by relating conventional, established techniques such as quantitative reverse transcription polymerase chain reaction (RT-qPCR), microarray, and Northern blotting (NB) and relatively recent advancements such as next-generation sequencing (NGS), highly sensitive biosensors, and computational prediction of microRNAtargets to common miR research strategies. This should guide interested readers toward a more focused study of miR research and the surrounding technology.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 217-237 |
| Number of pages | 21 |
| Journal | Annual Review of Analytical Chemistry |
| Volume | 8 |
| DOIs | |
| State | Published - Jul 22 2015 |
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Keywords
- Biosensors
- Microarray
- NcRNA
- Northern blot
- RNA-seq
- RT-qPCR
ASJC Scopus subject areas
- Analytical Chemistry
Cite this
MicroRNA Detection : Current Technology and Research Strategies. / Hunt, Eric A.; Broyles, David; Head, Trajen; Deo, Sapna K.
In: Annual Review of Analytical Chemistry, Vol. 8, 22.07.2015, p. 217-237.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MicroRNA Detection
T2 - Current Technology and Research Strategies
AU - Hunt, Eric A.
AU - Broyles, David
AU - Head, Trajen
AU - Deo, Sapna K
PY - 2015/7/22
Y1 - 2015/7/22
N2 - The relatively new field of microRNA (miR) has experienced rapid growth in methodology associated with its detection and bioanalysis as well as with its role in -omics research, clinical diagnostics, and new therapeutic strategies. The breadth of this area of research and the seemingly exponential increase in number of publications on the subject can present scientists new to the field with a daunting amount of information to evaluate. This review aims to provide a collective overview of miR detection methods by relating conventional, established techniques such as quantitative reverse transcription polymerase chain reaction (RT-qPCR), microarray, and Northern blotting (NB) and relatively recent advancements such as next-generation sequencing (NGS), highly sensitive biosensors, and computational prediction of microRNAtargets to common miR research strategies. This should guide interested readers toward a more focused study of miR research and the surrounding technology.
AB - The relatively new field of microRNA (miR) has experienced rapid growth in methodology associated with its detection and bioanalysis as well as with its role in -omics research, clinical diagnostics, and new therapeutic strategies. The breadth of this area of research and the seemingly exponential increase in number of publications on the subject can present scientists new to the field with a daunting amount of information to evaluate. This review aims to provide a collective overview of miR detection methods by relating conventional, established techniques such as quantitative reverse transcription polymerase chain reaction (RT-qPCR), microarray, and Northern blotting (NB) and relatively recent advancements such as next-generation sequencing (NGS), highly sensitive biosensors, and computational prediction of microRNAtargets to common miR research strategies. This should guide interested readers toward a more focused study of miR research and the surrounding technology.
KW - Biosensors
KW - Microarray
KW - NcRNA
KW - Northern blot
KW - RNA-seq
KW - RT-qPCR
UR - http://www.scopus.com/inward/record.url?scp=84937416195&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84937416195&partnerID=8YFLogxK
U2 - 10.1146/annurev-anchem-071114-040343
DO - 10.1146/annurev-anchem-071114-040343
M3 - Article
C2 - 25973944
AN - SCOPUS:84937416195
VL - 8
SP - 217
EP - 237
JO - Annual Review of Analytical Chemistry
JF - Annual Review of Analytical Chemistry
SN - 1936-1327
ER -