MicroRNA-148a-3p enhances cisplatin cytotoxicity in gastric cancer through mitochondrial fission induction and cyto-protective autophagy suppression

Bowen Li, Weizhi Wang, Zheng Li, Zheng Chen, Xiaofei Zhi, Jianghao Xu, Qing Li, Lu Wang, Xiaoxu Huang, Linjun Wang, Song Wei, Guangli Sun, Xuan Zhang, Zhongyuan He, Lu Zhang, Diancai Zhang, Hao Xu, Wael El-Rifai, Zekuan Xu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Cisplatin (CDDP) resistance is a major clinical problem associated with poor prognosis in gastric cancer (GC) patients. In this study, we performed integrated analysis of TCGA data from microRNAs (miRNAs) expression matrix of GC patients who received CDDP-based chemotherapy with GEO dataset which contains differential miRNAs expression profiles in CDDP-resistant and -sensitive cell lines. We identified miR-148a-3p downregulation as a key step involved in CDDP resistance. Using a cohort consisting 105 GC patients who received CDDP-based therapy, we found that miR-148a-3p downregulation was associated with a decrease in patients' disease-free survival (DFS, P = 0.0077). A series of experiment data demonstrated that: 1) miR-148a-3p was downregulated in CDDP-resistant GC cell lines; 2) miR-148a-3p reconstitution sensitized CDDP-resistant cells to CDDP treatment through promoting mitochondrial fission and decreasing AKAP1 expression level; 3) AKAP1 played a novel role in CDDP resistance by inhibiting P53-mediated DRP1 dephosphorylation; 4) miR-148a-3p reconstitution in CDDP-resistant cells inhibits the cyto-protective autophagy by suppressing RAB12 expression and mTOR1 activation. Taken together, our study demonstrates that miR-148a-3p could be a promising prognostic marker or therapeutic candidate for overcoming CDDP resistance in GC.

Original languageEnglish (US)
Pages (from-to)212-227
Number of pages16
JournalCancer Letters
Volume410
DOIs
StatePublished - Dec 1 2017
Externally publishedYes

Fingerprint

Mitochondrial Dynamics
Autophagy
MicroRNAs
Cisplatin
Stomach Neoplasms
Down-Regulation
Cell Line
Disease-Free Survival
Therapeutics
Drug Therapy

Keywords

  • AKAP1
  • Cisplatin sensitivity
  • Gastric cancer
  • miR-148a-3p
  • RAB12

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

MicroRNA-148a-3p enhances cisplatin cytotoxicity in gastric cancer through mitochondrial fission induction and cyto-protective autophagy suppression. / Li, Bowen; Wang, Weizhi; Li, Zheng; Chen, Zheng; Zhi, Xiaofei; Xu, Jianghao; Li, Qing; Wang, Lu; Huang, Xiaoxu; Wang, Linjun; Wei, Song; Sun, Guangli; Zhang, Xuan; He, Zhongyuan; Zhang, Lu; Zhang, Diancai; Xu, Hao; El-Rifai, Wael; Xu, Zekuan.

In: Cancer Letters, Vol. 410, 01.12.2017, p. 212-227.

Research output: Contribution to journalArticle

Li, B, Wang, W, Li, Z, Chen, Z, Zhi, X, Xu, J, Li, Q, Wang, L, Huang, X, Wang, L, Wei, S, Sun, G, Zhang, X, He, Z, Zhang, L, Zhang, D, Xu, H, El-Rifai, W & Xu, Z 2017, 'MicroRNA-148a-3p enhances cisplatin cytotoxicity in gastric cancer through mitochondrial fission induction and cyto-protective autophagy suppression', Cancer Letters, vol. 410, pp. 212-227. https://doi.org/10.1016/j.canlet.2017.09.035
Li, Bowen ; Wang, Weizhi ; Li, Zheng ; Chen, Zheng ; Zhi, Xiaofei ; Xu, Jianghao ; Li, Qing ; Wang, Lu ; Huang, Xiaoxu ; Wang, Linjun ; Wei, Song ; Sun, Guangli ; Zhang, Xuan ; He, Zhongyuan ; Zhang, Lu ; Zhang, Diancai ; Xu, Hao ; El-Rifai, Wael ; Xu, Zekuan. / MicroRNA-148a-3p enhances cisplatin cytotoxicity in gastric cancer through mitochondrial fission induction and cyto-protective autophagy suppression. In: Cancer Letters. 2017 ; Vol. 410. pp. 212-227.
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AU - Li, Bowen

AU - Wang, Weizhi

AU - Li, Zheng

AU - Chen, Zheng

AU - Zhi, Xiaofei

AU - Xu, Jianghao

AU - Li, Qing

AU - Wang, Lu

AU - Huang, Xiaoxu

AU - Wang, Linjun

AU - Wei, Song

AU - Sun, Guangli

AU - Zhang, Xuan

AU - He, Zhongyuan

AU - Zhang, Lu

AU - Zhang, Diancai

AU - Xu, Hao

AU - El-Rifai, Wael

AU - Xu, Zekuan

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N2 - Cisplatin (CDDP) resistance is a major clinical problem associated with poor prognosis in gastric cancer (GC) patients. In this study, we performed integrated analysis of TCGA data from microRNAs (miRNAs) expression matrix of GC patients who received CDDP-based chemotherapy with GEO dataset which contains differential miRNAs expression profiles in CDDP-resistant and -sensitive cell lines. We identified miR-148a-3p downregulation as a key step involved in CDDP resistance. Using a cohort consisting 105 GC patients who received CDDP-based therapy, we found that miR-148a-3p downregulation was associated with a decrease in patients' disease-free survival (DFS, P = 0.0077). A series of experiment data demonstrated that: 1) miR-148a-3p was downregulated in CDDP-resistant GC cell lines; 2) miR-148a-3p reconstitution sensitized CDDP-resistant cells to CDDP treatment through promoting mitochondrial fission and decreasing AKAP1 expression level; 3) AKAP1 played a novel role in CDDP resistance by inhibiting P53-mediated DRP1 dephosphorylation; 4) miR-148a-3p reconstitution in CDDP-resistant cells inhibits the cyto-protective autophagy by suppressing RAB12 expression and mTOR1 activation. Taken together, our study demonstrates that miR-148a-3p could be a promising prognostic marker or therapeutic candidate for overcoming CDDP resistance in GC.

AB - Cisplatin (CDDP) resistance is a major clinical problem associated with poor prognosis in gastric cancer (GC) patients. In this study, we performed integrated analysis of TCGA data from microRNAs (miRNAs) expression matrix of GC patients who received CDDP-based chemotherapy with GEO dataset which contains differential miRNAs expression profiles in CDDP-resistant and -sensitive cell lines. We identified miR-148a-3p downregulation as a key step involved in CDDP resistance. Using a cohort consisting 105 GC patients who received CDDP-based therapy, we found that miR-148a-3p downregulation was associated with a decrease in patients' disease-free survival (DFS, P = 0.0077). A series of experiment data demonstrated that: 1) miR-148a-3p was downregulated in CDDP-resistant GC cell lines; 2) miR-148a-3p reconstitution sensitized CDDP-resistant cells to CDDP treatment through promoting mitochondrial fission and decreasing AKAP1 expression level; 3) AKAP1 played a novel role in CDDP resistance by inhibiting P53-mediated DRP1 dephosphorylation; 4) miR-148a-3p reconstitution in CDDP-resistant cells inhibits the cyto-protective autophagy by suppressing RAB12 expression and mTOR1 activation. Taken together, our study demonstrates that miR-148a-3p could be a promising prognostic marker or therapeutic candidate for overcoming CDDP resistance in GC.

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