MicroRNA-141-3p Negatively Modulates SDF-1 Expression in Age-Dependent Pathophysiology of Human and Murine Bone Marrow Stromal Cells

Sudharsan Periyasamy-Thandavan, John Burke, Bharati Mendhe, Galina Kondrikova, Ravindra Kolhe, Monte Hunter, Carlos M. Isales, Mark W. Hamrick, William D. Hill, Sadanand Fulzele, Isabel Beerman, Joshua Hare

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Stromal cell-derived factor-1 (SDF-1 or CXCL12) is a cytokine secreted by cells including bone marrow stromal cells (BMSCs). SDF-1 plays a vital role in BMSC migration, survival, and differentiation. Our group previously reported the role of SDF-1 in osteogenic differentiation in vitro and bone formation in vivo; however, our understanding of the post-transcriptional regulatory mechanism of SDF-1 remains poor. MicroRNAs are small noncoding RNAs that post-transcriptionally regulate the messenger RNAs (mRNAs) of protein-coding genes. In this study, we aimed to investigate the impact of miR-141-3p on SDF-1 expression in BMSCs and its importance in the aging bone marrow (BM) microenvironment. Our data demonstrated that murine and human BMSCs expressed miR-141-3p that repressed SDF-1 gene expression at the functional level (luciferase reporter assay) by targeting the 3′-untranslated region of mRNA. We also found that transfection of miR-141-3p decreased osteogenic markers in human BMSCs. Our results demonstrate that miR-141-3p expression increases with age, while SDF-1 decreases in both the human and mouse BM niche. Taken together, these results support that miR-141-3p is a novel regulator of SDF-1 in bone cells and plays an important role in the age-dependent pathophysiology of murine and human BM niche.

Original languageEnglish (US)
Pages (from-to)1368-1374
Number of pages7
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume74
Issue number9
DOIs
StatePublished - Aug 16 2019

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MicroRNAs
Mesenchymal Stromal Cells
Bone Marrow
Chemokine CXCL12
Small Untranslated RNA
Messenger RNA
3' Untranslated Regions
Luciferases
Osteogenesis
Cell Movement
Transfection
Cell Differentiation
Cell Survival
Cytokines
Gene Expression
Bone and Bones
Proteins

Keywords

  • Aging
  • Bone marrow stromal cells
  • miR-141
  • SDF-1

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

MicroRNA-141-3p Negatively Modulates SDF-1 Expression in Age-Dependent Pathophysiology of Human and Murine Bone Marrow Stromal Cells. / Periyasamy-Thandavan, Sudharsan; Burke, John; Mendhe, Bharati; Kondrikova, Galina; Kolhe, Ravindra; Hunter, Monte; Isales, Carlos M.; Hamrick, Mark W.; Hill, William D.; Fulzele, Sadanand; Beerman, Isabel; Hare, Joshua.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 74, No. 9, 16.08.2019, p. 1368-1374.

Research output: Contribution to journalArticle

Periyasamy-Thandavan, S, Burke, J, Mendhe, B, Kondrikova, G, Kolhe, R, Hunter, M, Isales, CM, Hamrick, MW, Hill, WD, Fulzele, S, Beerman, I & Hare, J 2019, 'MicroRNA-141-3p Negatively Modulates SDF-1 Expression in Age-Dependent Pathophysiology of Human and Murine Bone Marrow Stromal Cells', Journals of Gerontology - Series A Biological Sciences and Medical Sciences, vol. 74, no. 9, pp. 1368-1374. https://doi.org/10.1093/gerona/gly186
Periyasamy-Thandavan, Sudharsan ; Burke, John ; Mendhe, Bharati ; Kondrikova, Galina ; Kolhe, Ravindra ; Hunter, Monte ; Isales, Carlos M. ; Hamrick, Mark W. ; Hill, William D. ; Fulzele, Sadanand ; Beerman, Isabel ; Hare, Joshua. / MicroRNA-141-3p Negatively Modulates SDF-1 Expression in Age-Dependent Pathophysiology of Human and Murine Bone Marrow Stromal Cells. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2019 ; Vol. 74, No. 9. pp. 1368-1374.
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AU - Mendhe, Bharati

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AU - Isales, Carlos M.

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AB - Stromal cell-derived factor-1 (SDF-1 or CXCL12) is a cytokine secreted by cells including bone marrow stromal cells (BMSCs). SDF-1 plays a vital role in BMSC migration, survival, and differentiation. Our group previously reported the role of SDF-1 in osteogenic differentiation in vitro and bone formation in vivo; however, our understanding of the post-transcriptional regulatory mechanism of SDF-1 remains poor. MicroRNAs are small noncoding RNAs that post-transcriptionally regulate the messenger RNAs (mRNAs) of protein-coding genes. In this study, we aimed to investigate the impact of miR-141-3p on SDF-1 expression in BMSCs and its importance in the aging bone marrow (BM) microenvironment. Our data demonstrated that murine and human BMSCs expressed miR-141-3p that repressed SDF-1 gene expression at the functional level (luciferase reporter assay) by targeting the 3′-untranslated region of mRNA. We also found that transfection of miR-141-3p decreased osteogenic markers in human BMSCs. Our results demonstrate that miR-141-3p expression increases with age, while SDF-1 decreases in both the human and mouse BM niche. Taken together, these results support that miR-141-3p is a novel regulator of SDF-1 in bone cells and plays an important role in the age-dependent pathophysiology of murine and human BM niche.

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