TY - JOUR
T1 - MicroRNA-132 directs human periodontal ligament-derived neural crest stem cell neural differentiation
AU - Ng, Tsz Kin
AU - Yang, Qichen
AU - Fortino, Veronica R.
AU - Lai, Nikky Yuk Ki
AU - Carballosa, Carlos M.
AU - Greenberg, Jordan M.
AU - Choy, Kwong Wai
AU - Pelaez, Daniel
AU - Pang, Chi Pui
AU - Cheung, Herman S.
N1 - Funding Information:
This work was supported in part by a block grant of the University Grants Committee Hong Kong and the Endowment Fund for Lim Por-Yen Eye Genetics Research Centre, Hong Kong, and a Veterans Affairs (VA) Merit Review Grant (to H.?S.?C.), and a Senior VA Research Career Scientist Award (to H.?S.?C.), Miami.
Funding Information:
Senior VA Research Career Scientist Award; Veterans Affairs (VA) Merit Review Grant; University Grants Committee Hong Kong
Funding Information:
This work was supported in part by a block grant of the University Grants Committee Hong Kong and the Endowment Fund for Lim Por‐Yen Eye Genetics Research Centre, Hong Kong, and a Veterans Affairs (VA) Merit Review Grant (to H. S. C.), and a Senior VA Research Career Scientist Award (to H. S. C.), Miami.
Publisher Copyright:
© 2018 John Wiley & Sons, Ltd.
PY - 2019/1
Y1 - 2019/1
N2 - Neurogenesis is the basis of stem cell tissue engineering and regenerative medicine for central nervous system (CNS) disorders. We have established differentiation protocols to direct human periodontal ligament-derived stem cells (PDLSCs) into neuronal lineage, and we recently isolated the neural crest subpopulation from PDLSCs, which are pluripotent in nature. Here, we report the neural differentiation potential of these periodontal ligament-derived neural crest stem cells (NCSCs) as well as its microRNA (miRNA) regulatory mechanism and function in NCSC neural differentiation. NCSCs, treated with basic fibroblast growth factor and epidermal growth factor-based differentiation medium for 24 days, expressed neuronal and glial markers (βIII-tubulin, neurofilament, NeuN, neuron-specific enolase, GFAP, and S100) and exhibited glutamate-induced calcium responses. The global miRNA expression profiling identified 60 upregulated and 19 downregulated human miRNAs after neural differentiation, and the gene ontology analysis of the miRNA target genes confirmed the neuronal differentiation-related biological functions. In addition, overexpression of miR-132 in NCSCs promoted the expression of neuronal markers and downregulated ZEB2 protein expression. Our results suggested that the pluripotent NCSCs from human periodontal ligament can be directed into neural lineage, which demonstrate its potential in tissue engineering and regenerative medicine for CNS disorders.
AB - Neurogenesis is the basis of stem cell tissue engineering and regenerative medicine for central nervous system (CNS) disorders. We have established differentiation protocols to direct human periodontal ligament-derived stem cells (PDLSCs) into neuronal lineage, and we recently isolated the neural crest subpopulation from PDLSCs, which are pluripotent in nature. Here, we report the neural differentiation potential of these periodontal ligament-derived neural crest stem cells (NCSCs) as well as its microRNA (miRNA) regulatory mechanism and function in NCSC neural differentiation. NCSCs, treated with basic fibroblast growth factor and epidermal growth factor-based differentiation medium for 24 days, expressed neuronal and glial markers (βIII-tubulin, neurofilament, NeuN, neuron-specific enolase, GFAP, and S100) and exhibited glutamate-induced calcium responses. The global miRNA expression profiling identified 60 upregulated and 19 downregulated human miRNAs after neural differentiation, and the gene ontology analysis of the miRNA target genes confirmed the neuronal differentiation-related biological functions. In addition, overexpression of miR-132 in NCSCs promoted the expression of neuronal markers and downregulated ZEB2 protein expression. Our results suggested that the pluripotent NCSCs from human periodontal ligament can be directed into neural lineage, which demonstrate its potential in tissue engineering and regenerative medicine for CNS disorders.
KW - ZEB2
KW - microRNAs
KW - neural crest stem cells
KW - neural differentiation
KW - neurons
KW - periodontal ligament
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U2 - 10.1002/term.2759
DO - 10.1002/term.2759
M3 - Article
C2 - 30352481
AN - SCOPUS:85058670062
VL - 13
SP - 12
EP - 24
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 1
ER -