Abstract
Poly(propylene sulfide-bl-ethylene glycol) (PPS-PEG) is an amphiphilic block copolymer that spontaneously adsorbs onto gold from solution. This results in the formation of a stable polymeric layer that renders the surface protein-resistant when an appropriate architecture is chosen. The established molecular-assembly patterning by lift-off (MAPL) technique can convert a prestructured resist film into a pattern of biointeractive chemistry and a non-interactive background. Employing the MAPL technique, we produced a micron-scale PPS-PEG pattern on a gold substrate, and then characterized the patterned structure with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and atomic force microscopy (AFM). Subsequent exposure of the PPS-PEG/gold pattern to protein adsorption (full human serum) was monitored in situ; SPR-imaging (i-SPR) shows a selective adsorption of proteins on gold, but not on PPS-PEG areas. Analysis shows a reduction of serum adsorption up to 93% on the PPS-PEG areas as compared to gold, in good agreement with previous analysis of homogenously adsorbed PPS-PEG on gold. MAPL patterning of PPS-PEG block copolymers is straightforward, versatile and reproducible, and may be incorporated into biosensor-based surface analysis methods.
| Original language | English |
|---|---|
| Pages (from-to) | 2305-2310 |
| Number of pages | 6 |
| Journal | Surface Science |
| Volume | 602 |
| Issue number | 13 |
| DOIs | |
| State | Published - Jul 1 2008 |
| Externally published | Yes |
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Keywords
- Adsorption
- Biosensing
- Gold
- Patterning
- Photolithography
- Self-assembly
- Surface plasmon techniques
- ToF-SIMS
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Condensed Matter Physics
- Surfaces and Interfaces
Cite this
Micropatterning of gold substrates based on poly(propylene sulfide-bl-ethylene glycol), (PPS-PEG) background passivation and the molecular-assembly patterning by lift-off (MAPL) technique. / Feller, L.; Bearinger, J. P.; Wu, L.; Hubbell, J. A.; Textor, M.; Tosatti, S.
In: Surface Science, Vol. 602, No. 13, 01.07.2008, p. 2305-2310.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Micropatterning of gold substrates based on poly(propylene sulfide-bl-ethylene glycol), (PPS-PEG) background passivation and the molecular-assembly patterning by lift-off (MAPL) technique
AU - Feller, L.
AU - Bearinger, J. P.
AU - Wu, L.
AU - Hubbell, J. A.
AU - Textor, M.
AU - Tosatti, S.
PY - 2008/7/1
Y1 - 2008/7/1
N2 - Poly(propylene sulfide-bl-ethylene glycol) (PPS-PEG) is an amphiphilic block copolymer that spontaneously adsorbs onto gold from solution. This results in the formation of a stable polymeric layer that renders the surface protein-resistant when an appropriate architecture is chosen. The established molecular-assembly patterning by lift-off (MAPL) technique can convert a prestructured resist film into a pattern of biointeractive chemistry and a non-interactive background. Employing the MAPL technique, we produced a micron-scale PPS-PEG pattern on a gold substrate, and then characterized the patterned structure with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and atomic force microscopy (AFM). Subsequent exposure of the PPS-PEG/gold pattern to protein adsorption (full human serum) was monitored in situ; SPR-imaging (i-SPR) shows a selective adsorption of proteins on gold, but not on PPS-PEG areas. Analysis shows a reduction of serum adsorption up to 93% on the PPS-PEG areas as compared to gold, in good agreement with previous analysis of homogenously adsorbed PPS-PEG on gold. MAPL patterning of PPS-PEG block copolymers is straightforward, versatile and reproducible, and may be incorporated into biosensor-based surface analysis methods.
AB - Poly(propylene sulfide-bl-ethylene glycol) (PPS-PEG) is an amphiphilic block copolymer that spontaneously adsorbs onto gold from solution. This results in the formation of a stable polymeric layer that renders the surface protein-resistant when an appropriate architecture is chosen. The established molecular-assembly patterning by lift-off (MAPL) technique can convert a prestructured resist film into a pattern of biointeractive chemistry and a non-interactive background. Employing the MAPL technique, we produced a micron-scale PPS-PEG pattern on a gold substrate, and then characterized the patterned structure with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and atomic force microscopy (AFM). Subsequent exposure of the PPS-PEG/gold pattern to protein adsorption (full human serum) was monitored in situ; SPR-imaging (i-SPR) shows a selective adsorption of proteins on gold, but not on PPS-PEG areas. Analysis shows a reduction of serum adsorption up to 93% on the PPS-PEG areas as compared to gold, in good agreement with previous analysis of homogenously adsorbed PPS-PEG on gold. MAPL patterning of PPS-PEG block copolymers is straightforward, versatile and reproducible, and may be incorporated into biosensor-based surface analysis methods.
KW - Adsorption
KW - Biosensing
KW - Gold
KW - Patterning
KW - Photolithography
KW - Self-assembly
KW - Surface plasmon techniques
KW - ToF-SIMS
UR - http://www.scopus.com/inward/record.url?scp=45949100349&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45949100349&partnerID=8YFLogxK
U2 - 10.1016/j.susc.2008.05.010
DO - 10.1016/j.susc.2008.05.010
M3 - Article
AN - SCOPUS:45949100349
VL - 602
SP - 2305
EP - 2310
JO - Surface Science
JF - Surface Science
SN - 0039-6028
IS - 13
ER -