Abstract
Poly(propylene sulfide-bl-ethylene glycol) (PPS-PEG) is an amphiphilic block copolymer that spontaneously adsorbs onto gold from solution. This results in the formation of a stable polymeric layer that renders the surface protein-resistant when an appropriate architecture is chosen. The established molecular-assembly patterning by lift-off (MAPL) technique can convert a prestructured resist film into a pattern of biointeractive chemistry and a non-interactive background. Employing the MAPL technique, we produced a micron-scale PPS-PEG pattern on a gold substrate, and then characterized the patterned structure with time-of-flight secondary ion mass spectrometry (ToF-SIMS) and atomic force microscopy (AFM). Subsequent exposure of the PPS-PEG/gold pattern to protein adsorption (full human serum) was monitored in situ; SPR-imaging (i-SPR) shows a selective adsorption of proteins on gold, but not on PPS-PEG areas. Analysis shows a reduction of serum adsorption up to 93% on the PPS-PEG areas as compared to gold, in good agreement with previous analysis of homogenously adsorbed PPS-PEG on gold. MAPL patterning of PPS-PEG block copolymers is straightforward, versatile and reproducible, and may be incorporated into biosensor-based surface analysis methods.
Original language | English (US) |
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Pages (from-to) | 2305-2310 |
Number of pages | 6 |
Journal | Surface Science |
Volume | 602 |
Issue number | 13 |
DOIs | |
State | Published - Jul 1 2008 |
Keywords
- Adsorption
- Biosensing
- Gold
- Patterning
- Photolithography
- Self-assembly
- Surface plasmon techniques
- ToF-SIMS
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Condensed Matter Physics
- Surfaces and Interfaces