Micron scale electromagnetic viable tumor cell capture and release

Yagmur Akin Yildirim, Burak Yildirim, Tan A. Ince, Onur Tigli

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we report on new developments, improved results and a new fabrication approach to a micro-electromagnet-based microfluidic device capable of capturing and releasing superparamagnetic beads and bead-bound MCF-7 cancer cells on a sensor site with high efficiency based on the challenges identified and reported in our preliminary studies (Gajasinghe et al. 2016). The device is biocompatible and fabricated by utilizing only traditional microfabrication methods. The microfluidic channel is coated with SU-8 to provide uniform and smooth channel surface for easy release of the captured viable cells without any chemical usage. We labelled MCF-7 cells with superparamagnetic beads via Ep-CAM antibody and observed capture rates of the superparamagnetic beads and MCF-7 cells are 99.9 ± 0.2%, and 94.4 ± 7%, and release efficiencies are 97.4 ± 2%, and 95.2 ± 3%, respectively. Furthermore, there was no significant degrade on viability was observed. Reusability of the device was also presented. Overall, our device is highly efficient, fast, low cost, and has potential be used for cell analysis, drug screening, and disease diagnostics.

Original languageEnglish (US)
Article number75
JournalMicrofluidics and Nanofluidics
Volume24
Issue number10
DOIs
StatePublished - Oct 1 2020
Externally publishedYes

Keywords

  • Cell capture and release
  • Circulating tumor cells
  • Electromagnetic cell capture
  • Lab-on-a-chip
  • Micro-electromagnets
  • Single cell analysis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Micron scale electromagnetic viable tumor cell capture and release'. Together they form a unique fingerprint.

Cite this