Micromolded gelatin hydrogels crosslinked with laminin for disease on a chip applications

Rachel Besser, Renata Maciel, Michael Dvornik, Daniel Carbonero, Isabella Claure, Ellery Jones, Joseph Reda, Mario Saporta, Ashutosh Agarwal

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: In recent years, biomimetic extracellular matrices (ECM) have been engineered to recapitulate the in vivo environment including proper elastic moduli, pore size, and major ECM proteins. One such protein is laminin, a high molecular weight protein found in the basal lamina of most biological tissues. The development of an easy to fabricate biomaterial with a functionalized laminin layer would provide proper focal adhesion points, allowing for improved culture and enhanced maturation of many cell types. Gelatin is a widely-used alternative to collagen, a major ECM structural protein present in many tissues. Gelatin is inexpensive, readily available, adhesive to many cell types, contains ideal mechanical properties and can be functionalized using various fabrication techniques. A laminin functionalized gelatin hydrogel would further properly mimic the ECM found in many organ systems, while providing proper mechanical and chemical cues to the cells. In this study, we used a gelatin-laminin (Gel-LN) hydrogel to create a 2D in vitro model of the neuromuscular junction.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Besser, R., Maciel, R., Dvornik, M., Carbonero, D., Claure, I., Jones, E., Reda, J., Saporta, M., & Agarwal, A. (2019). Micromolded gelatin hydrogels crosslinked with laminin for disease on a chip applications. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.