Micromolded gelatin hydrogels crosslinked with laminin for disease on a chip applications

Rachel Besser, Renata Maciel, Michael Dvornik, Daniel Carbonero, Isabella Claure, Ellery Jones, Joseph Reda, Mario da Cunha Saporta, Ashutosh Agarwal

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: In recent years, biomimetic extracellular matrices (ECM) have been engineered to recapitulate the in vivo environment including proper elastic moduli, pore size, and major ECM proteins. One such protein is laminin, a high molecular weight protein found in the basal lamina of most biological tissues. The development of an easy to fabricate biomaterial with a functionalized laminin layer would provide proper focal adhesion points, allowing for improved culture and enhanced maturation of many cell types. Gelatin is a widely-used alternative to collagen, a major ECM structural protein present in many tissues. Gelatin is inexpensive, readily available, adhesive to many cell types, contains ideal mechanical properties and can be functionalized using various fabrication techniques. A laminin functionalized gelatin hydrogel would further properly mimic the ECM found in many organ systems, while providing proper mechanical and chemical cues to the cells. In this study, we used a gelatin-laminin (Gel-LN) hydrogel to create a 2D in vitro model of the neuromuscular junction.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

Fingerprint

Hydrogels
Laminin
Gelatin
Proteins
Extracellular Matrix Proteins
Hydrogel
Extracellular Matrix
Tissue
Biomimetics
Focal Adhesions
Neuromuscular Junction
Elastic Modulus
Biocompatible Materials
Basement Membrane
Collagen
Biomaterials
Adhesives
Pore size
Cues
Adhesion

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Besser, R., Maciel, R., Dvornik, M., Carbonero, D., Claure, I., Jones, E., ... Agarwal, A. (2019). Micromolded gelatin hydrogels crosslinked with laminin for disease on a chip applications. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.

Micromolded gelatin hydrogels crosslinked with laminin for disease on a chip applications. / Besser, Rachel; Maciel, Renata; Dvornik, Michael; Carbonero, Daniel; Claure, Isabella; Jones, Ellery; Reda, Joseph; da Cunha Saporta, Mario; Agarwal, Ashutosh.

Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Besser, R, Maciel, R, Dvornik, M, Carbonero, D, Claure, I, Jones, E, Reda, J, da Cunha Saporta, M & Agarwal, A 2019, Micromolded gelatin hydrogels crosslinked with laminin for disease on a chip applications. in Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, vol. 40, Society for Biomaterials, 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence, Seattle, United States, 4/3/19.
Besser R, Maciel R, Dvornik M, Carbonero D, Claure I, Jones E et al. Micromolded gelatin hydrogels crosslinked with laminin for disease on a chip applications. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials. 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
Besser, Rachel ; Maciel, Renata ; Dvornik, Michael ; Carbonero, Daniel ; Claure, Isabella ; Jones, Ellery ; Reda, Joseph ; da Cunha Saporta, Mario ; Agarwal, Ashutosh. / Micromolded gelatin hydrogels crosslinked with laminin for disease on a chip applications. Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
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