Statement of Purpose: In recent years, biomimetic extracellular matrices (ECM) have been engineered to recapitulate the in vivo environment including proper elastic moduli, pore size, and major ECM proteins. One such protein is laminin, a high molecular weight protein found in the basal lamina of most biological tissues. The development of an easy to fabricate biomaterial with a functionalized laminin layer would provide proper focal adhesion points, allowing for improved culture and enhanced maturation of many cell types. Gelatin is a widely-used alternative to collagen, a major ECM structural protein present in many tissues. Gelatin is inexpensive, readily available, adhesive to many cell types, contains ideal mechanical properties and can be functionalized using various fabrication techniques. A laminin functionalized gelatin hydrogel would further properly mimic the ECM found in many organ systems, while providing proper mechanical and chemical cues to the cells. In this study, we used a gelatin-laminin (Gel-LN) hydrogel to create a 2D in vitro model of the neuromuscular junction.