Abstract
Microinjection of Xenopus oocytes has proven to be a valuable tool in a broad array of studies that require expression of DNA or RNA into functional protein. These studies are diverse and range from expression cloning to receptor–ligand interaction to nuclear programming. Oocytes offer a number of advantages for such studies, including their large size (~1.2 mm in diameter), capacity for translation, and enormous nucleus (0.3–0.4 mm). They are cost effective, easily manipulated, and can be injected in large numbers in a short time period. Oocytes have a large maternal stockpile of all the essential components for transcription and translation. Consequently, the investigator needs only to introduce by microinjection the specific DNA or RNA of interest for synthesis. Oocytes translate virtually any exogenous RNA regardless of source, and the translated proteins are folded, modified, and transported to the correct cellular locations. Here we present procedures for the efficient microinjection of oocytes and their subsequent care.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 92-98 |
| Number of pages | 7 |
| Journal | Cold Spring Harbor Protocols |
| Volume | 2018 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2018 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Microinjection of xenopus oocytes. / Aguero, Tristan; Newman, Karen; King, Mary Lou L.
In: Cold Spring Harbor Protocols, Vol. 2018, No. 2, 01.02.2018, p. 92-98.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microinjection of xenopus oocytes
AU - Aguero, Tristan
AU - Newman, Karen
AU - King, Mary Lou L
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Microinjection of Xenopus oocytes has proven to be a valuable tool in a broad array of studies that require expression of DNA or RNA into functional protein. These studies are diverse and range from expression cloning to receptor–ligand interaction to nuclear programming. Oocytes offer a number of advantages for such studies, including their large size (~1.2 mm in diameter), capacity for translation, and enormous nucleus (0.3–0.4 mm). They are cost effective, easily manipulated, and can be injected in large numbers in a short time period. Oocytes have a large maternal stockpile of all the essential components for transcription and translation. Consequently, the investigator needs only to introduce by microinjection the specific DNA or RNA of interest for synthesis. Oocytes translate virtually any exogenous RNA regardless of source, and the translated proteins are folded, modified, and transported to the correct cellular locations. Here we present procedures for the efficient microinjection of oocytes and their subsequent care.
AB - Microinjection of Xenopus oocytes has proven to be a valuable tool in a broad array of studies that require expression of DNA or RNA into functional protein. These studies are diverse and range from expression cloning to receptor–ligand interaction to nuclear programming. Oocytes offer a number of advantages for such studies, including their large size (~1.2 mm in diameter), capacity for translation, and enormous nucleus (0.3–0.4 mm). They are cost effective, easily manipulated, and can be injected in large numbers in a short time period. Oocytes have a large maternal stockpile of all the essential components for transcription and translation. Consequently, the investigator needs only to introduce by microinjection the specific DNA or RNA of interest for synthesis. Oocytes translate virtually any exogenous RNA regardless of source, and the translated proteins are folded, modified, and transported to the correct cellular locations. Here we present procedures for the efficient microinjection of oocytes and their subsequent care.
UR - http://www.scopus.com/inward/record.url?scp=85041445505&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85041445505&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot096974
DO - 10.1101/pdb.prot096974
M3 - Article
AN - SCOPUS:85041445505
VL - 2018
SP - 92
EP - 98
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
SN - 1559-6095
IS - 2
ER -