Microinjection of xenopus oocytes

Tristan Aguero, Karen Newman, Mary Lou L King

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microinjection of Xenopus oocytes has proven to be a valuable tool in a broad array of studies that require expression of DNA or RNA into functional protein. These studies are diverse and range from expression cloning to receptor–ligand interaction to nuclear programming. Oocytes offer a number of advantages for such studies, including their large size (~1.2 mm in diameter), capacity for translation, and enormous nucleus (0.3–0.4 mm). They are cost effective, easily manipulated, and can be injected in large numbers in a short time period. Oocytes have a large maternal stockpile of all the essential components for transcription and translation. Consequently, the investigator needs only to introduce by microinjection the specific DNA or RNA of interest for synthesis. Oocytes translate virtually any exogenous RNA regardless of source, and the translated proteins are folded, modified, and transported to the correct cellular locations. Here we present procedures for the efficient microinjection of oocytes and their subsequent care.

Original languageEnglish (US)
Pages (from-to)92-98
Number of pages7
JournalCold Spring Harbor Protocols
Volume2018
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Microinjections
Xenopus
Oocytes
RNA
Cloning
DNA
Transcription
Proteins
Organism Cloning
Mothers
Research Personnel
Costs
Costs and Cost Analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Aguero, T., Newman, K., & King, M. L. L. (2018). Microinjection of xenopus oocytes. Cold Spring Harbor Protocols, 2018(2), 92-98. https://doi.org/10.1101/pdb.prot096974

Microinjection of xenopus oocytes. / Aguero, Tristan; Newman, Karen; King, Mary Lou L.

In: Cold Spring Harbor Protocols, Vol. 2018, No. 2, 01.02.2018, p. 92-98.

Research output: Contribution to journalArticle

Aguero, T, Newman, K & King, MLL 2018, 'Microinjection of xenopus oocytes', Cold Spring Harbor Protocols, vol. 2018, no. 2, pp. 92-98. https://doi.org/10.1101/pdb.prot096974
Aguero, Tristan ; Newman, Karen ; King, Mary Lou L. / Microinjection of xenopus oocytes. In: Cold Spring Harbor Protocols. 2018 ; Vol. 2018, No. 2. pp. 92-98.
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