Microinjection of xenopus oocytes

Tristan Aguero, Karen Newman, Mary Lou L King

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Microinjection of Xenopus oocytes has proven to be a valuable tool in a broad array of studies that require expression of DNA or RNA into functional protein. These studies are diverse and range from expression cloning to receptor–ligand interaction to nuclear programming. Oocytes offer a number of advantages for such studies, including their large size (~1.2 mm in diameter), capacity for translation, and enormous nucleus (0.3–0.4 mm). They are cost effective, easily manipulated, and can be injected in large numbers in a short time period. Oocytes have a large maternal stockpile of all the essential components for transcription and translation. Consequently, the investigator needs only to introduce by microinjection the specific DNA or RNA of interest for synthesis. Oocytes translate virtually any exogenous RNA regardless of source, and the translated proteins are folded, modified, and transported to the correct cellular locations. Here we present procedures for the efficient microinjection of oocytes and their subsequent care.

Original languageEnglish (US)
Pages (from-to)92-98
Number of pages7
JournalCold Spring Harbor Protocols
Volume2018
Issue number2
DOIs
StatePublished - Feb 1 2018

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Aguero, T., Newman, K., & King, M. L. L. (2018). Microinjection of xenopus oocytes. Cold Spring Harbor Protocols, 2018(2), 92-98. https://doi.org/10.1101/pdb.prot096974