Microheterogeneity of enzymes and deactivation

Ajit Sadana, Arun Malhotra

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


The influence of microheterogeneity on enzyme inactivation kinetics is presented. Examples of different enzymes are given where microheterogeneity has been detected by different techniques. The different statistical models are presented which include the influence of microheterogeneity on enzyme inactivation kinetics and stability. As the microheterogeneity of the enzyme increases, there is a sharper decline in the normalized activity during the initial stages of the deactivation but a greater stability and activity, compared to similar homogeneous enzyme, as the deactivation proceeds. Microheterogeneity makes the deactivation reaction have a higher apparent order of reaction. The implications of microheterogeneity on enzyme inactivations are highlighted by different examples. The analysis provides fresh physical insights into the chemistry, sub-populations, structure, and function of enzymes.

Original languageEnglish (US)
Pages (from-to)1041-1056
Number of pages16
JournalBiotechnology and Bioengineering
Issue number9
StatePublished - Dec 20 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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