Microfluidic heart on a chip for higher throughput pharmacological studies

Ashutosh Agarwal, Josue Adrian Goss, Alexander Cho, Megan Laura McCain, Kevin Kit Parker

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

We present the design of a higher throughput "heart on a chip" which utilizes a semi-automated fabrication technique to process sub millimeter sized thin film cantilevers of soft elastomers. Anisotropic cardiac microtissues which recapitulate the laminar architecture of the heart ventricle are engineered on these cantilevers. Deflection of these cantilevers, termed Muscular Thin Films (MTFs), during muscle contraction allows calculation of diastolic and systolic stresses generated by the engineered tissues. We also present the design of a reusable one channel fluidic microdevice completely built out of autoclavable materials which incorporates various features required for an optical cardiac contractility assay: metallic base which fits on a heating element for temperature control, transparent top for recording cantilever deformation and embedded electrodes for electrical field stimulation of the tissue. We employ the microdevice to test the positive inotropic effect of isoproterenol on cardiac contractility at dosages ranging from 1 nM to 100 μM. The higher throughput fluidic heart on a chip has applications in testing of cardiac tissues built from rare or expensive cell sources and for integration with other organ mimics. These advances will help alleviate translational barriers for commercial adoption of these technologies by improving the throughput and reproducibility of readout, standardization of the platform and scalability of manufacture.

Original languageEnglish (US)
Pages (from-to)3599-3608
Number of pages10
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume13
Issue number18
DOIs
StatePublished - Sep 21 2013
Externally publishedYes

Fingerprint

Microfluidics
Throughput
Fluidics
Pharmacology
Tissue
Elastomers
Electric heating elements
Thin films
Muscle Contraction
Isoproterenol
Temperature control
Heating
Standardization
Electric Stimulation
Heart Ventricles
Muscle
Scalability
Assays
Electrodes
Technology

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Microfluidic heart on a chip for higher throughput pharmacological studies. / Agarwal, Ashutosh; Goss, Josue Adrian; Cho, Alexander; McCain, Megan Laura; Parker, Kevin Kit.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 13, No. 18, 21.09.2013, p. 3599-3608.

Research output: Contribution to journalArticle

Agarwal, Ashutosh ; Goss, Josue Adrian ; Cho, Alexander ; McCain, Megan Laura ; Parker, Kevin Kit. / Microfluidic heart on a chip for higher throughput pharmacological studies. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2013 ; Vol. 13, No. 18. pp. 3599-3608.
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