Microencapsulation of islets within alginate/poly(ethylene glycol) gels cross-linked via Staudinger ligation

K. K. Hall, K. M. Gattás-Asfura, C. L. Stabler

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Functionalized alginate and poly(ethylene glycol) (PEG) polymers were used to generate covalently linked alginate-PEG (XAlgPEG) microbeads of high stability. The cell-compatible Staudinger ligation scheme was used to cross-link phosphine-terminated PEG chemoselectively to azide-functionalized alginate, resulting in XAlgPEG hydrogels. XAlgPEG microbeads were formed by co-incubation of the two polymers, followed by ionic cross-linking of the alginate using barium ions. The enhanced stability and gel properties of the resulting XAlgPEG microbeads, as well as the compatibility of these polymers for the encapsulation of islets and beta cells lines, were investigated. The data show that XAlgPEG microbeads exhibit superior resistance to osmotic swelling compared with traditional barium cross-linked alginate (Ba-Alg) beads, with a five-fold reduction in observed swelling, as well as resistance to dissolution via chelation solution. Diffusion and porosity studies found XAlgPEG beads to exhibit properties comparable with standard Ba-Alg. XAlgPEG microbeads were found to be highly cell compatible with insulinoma cell lines, as well as rat and human pancreatic islets, where the viability and functional assessment of cells within XAlgPEG are comparable with Ba-Alg controls. The remarkable improved stability, as well as demonstrated cellular compatibility, of XAlgPEG hydrogels makes them an appealing option for a wide variety of tissue engineering applications.

Original languageEnglish
Pages (from-to)614-624
Number of pages11
JournalActa Biomaterialia
Volume7
Issue number2
DOIs
StatePublished - Feb 1 2011

Fingerprint

Microencapsulation
Drug Compounding
Ethylene Glycol
Alginate
Polyethylene glycols
Ligation
Gels
Microspheres
Barium
Polymers
phosphine
Hydrogels
Islets of Langerhans
Swelling
Functional assessment
Cells
Cell Line
Insulinoma
Azides
Porosity

Keywords

  • Alginate
  • Bioartificial pancreas
  • Cross-linking
  • Islet encapsulation
  • Staudinger ligation

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Microencapsulation of islets within alginate/poly(ethylene glycol) gels cross-linked via Staudinger ligation. / Hall, K. K.; Gattás-Asfura, K. M.; Stabler, C. L.

In: Acta Biomaterialia, Vol. 7, No. 2, 01.02.2011, p. 614-624.

Research output: Contribution to journalArticle

Hall, K. K. ; Gattás-Asfura, K. M. ; Stabler, C. L. / Microencapsulation of islets within alginate/poly(ethylene glycol) gels cross-linked via Staudinger ligation. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 2. pp. 614-624.
@article{013cfa1543fd4b0e92cea8b6743393fd,
title = "Microencapsulation of islets within alginate/poly(ethylene glycol) gels cross-linked via Staudinger ligation",
abstract = "Functionalized alginate and poly(ethylene glycol) (PEG) polymers were used to generate covalently linked alginate-PEG (XAlgPEG) microbeads of high stability. The cell-compatible Staudinger ligation scheme was used to cross-link phosphine-terminated PEG chemoselectively to azide-functionalized alginate, resulting in XAlgPEG hydrogels. XAlgPEG microbeads were formed by co-incubation of the two polymers, followed by ionic cross-linking of the alginate using barium ions. The enhanced stability and gel properties of the resulting XAlgPEG microbeads, as well as the compatibility of these polymers for the encapsulation of islets and beta cells lines, were investigated. The data show that XAlgPEG microbeads exhibit superior resistance to osmotic swelling compared with traditional barium cross-linked alginate (Ba-Alg) beads, with a five-fold reduction in observed swelling, as well as resistance to dissolution via chelation solution. Diffusion and porosity studies found XAlgPEG beads to exhibit properties comparable with standard Ba-Alg. XAlgPEG microbeads were found to be highly cell compatible with insulinoma cell lines, as well as rat and human pancreatic islets, where the viability and functional assessment of cells within XAlgPEG are comparable with Ba-Alg controls. The remarkable improved stability, as well as demonstrated cellular compatibility, of XAlgPEG hydrogels makes them an appealing option for a wide variety of tissue engineering applications.",
keywords = "Alginate, Bioartificial pancreas, Cross-linking, Islet encapsulation, Staudinger ligation",
author = "Hall, {K. K.} and Gatt{\'a}s-Asfura, {K. M.} and Stabler, {C. L.}",
year = "2011",
month = "2",
day = "1",
doi = "10.1016/j.actbio.2010.07.016",
language = "English",
volume = "7",
pages = "614--624",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Microencapsulation of islets within alginate/poly(ethylene glycol) gels cross-linked via Staudinger ligation

AU - Hall, K. K.

AU - Gattás-Asfura, K. M.

AU - Stabler, C. L.

PY - 2011/2/1

Y1 - 2011/2/1

N2 - Functionalized alginate and poly(ethylene glycol) (PEG) polymers were used to generate covalently linked alginate-PEG (XAlgPEG) microbeads of high stability. The cell-compatible Staudinger ligation scheme was used to cross-link phosphine-terminated PEG chemoselectively to azide-functionalized alginate, resulting in XAlgPEG hydrogels. XAlgPEG microbeads were formed by co-incubation of the two polymers, followed by ionic cross-linking of the alginate using barium ions. The enhanced stability and gel properties of the resulting XAlgPEG microbeads, as well as the compatibility of these polymers for the encapsulation of islets and beta cells lines, were investigated. The data show that XAlgPEG microbeads exhibit superior resistance to osmotic swelling compared with traditional barium cross-linked alginate (Ba-Alg) beads, with a five-fold reduction in observed swelling, as well as resistance to dissolution via chelation solution. Diffusion and porosity studies found XAlgPEG beads to exhibit properties comparable with standard Ba-Alg. XAlgPEG microbeads were found to be highly cell compatible with insulinoma cell lines, as well as rat and human pancreatic islets, where the viability and functional assessment of cells within XAlgPEG are comparable with Ba-Alg controls. The remarkable improved stability, as well as demonstrated cellular compatibility, of XAlgPEG hydrogels makes them an appealing option for a wide variety of tissue engineering applications.

AB - Functionalized alginate and poly(ethylene glycol) (PEG) polymers were used to generate covalently linked alginate-PEG (XAlgPEG) microbeads of high stability. The cell-compatible Staudinger ligation scheme was used to cross-link phosphine-terminated PEG chemoselectively to azide-functionalized alginate, resulting in XAlgPEG hydrogels. XAlgPEG microbeads were formed by co-incubation of the two polymers, followed by ionic cross-linking of the alginate using barium ions. The enhanced stability and gel properties of the resulting XAlgPEG microbeads, as well as the compatibility of these polymers for the encapsulation of islets and beta cells lines, were investigated. The data show that XAlgPEG microbeads exhibit superior resistance to osmotic swelling compared with traditional barium cross-linked alginate (Ba-Alg) beads, with a five-fold reduction in observed swelling, as well as resistance to dissolution via chelation solution. Diffusion and porosity studies found XAlgPEG beads to exhibit properties comparable with standard Ba-Alg. XAlgPEG microbeads were found to be highly cell compatible with insulinoma cell lines, as well as rat and human pancreatic islets, where the viability and functional assessment of cells within XAlgPEG are comparable with Ba-Alg controls. The remarkable improved stability, as well as demonstrated cellular compatibility, of XAlgPEG hydrogels makes them an appealing option for a wide variety of tissue engineering applications.

KW - Alginate

KW - Bioartificial pancreas

KW - Cross-linking

KW - Islet encapsulation

KW - Staudinger ligation

UR - http://www.scopus.com/inward/record.url?scp=78650735925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650735925&partnerID=8YFLogxK

U2 - 10.1016/j.actbio.2010.07.016

DO - 10.1016/j.actbio.2010.07.016

M3 - Article

C2 - 20654745

AN - SCOPUS:78650735925

VL - 7

SP - 614

EP - 624

JO - Acta Biomaterialia

JF - Acta Biomaterialia

SN - 1742-7061

IS - 2

ER -